Addgene

We narrowed to 4,144 results for: Pet2;

Showing: 3691 - 3720 of 4144 results

Results per page:

Sort results by:

pET-PLS-KP1_rpoA-U

Plasmid

#190955

Purpose

Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP1

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP2-A66S_rpoA-U

Plasmid

#190957

Purpose

Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP3-A66S_rpoA-U

Plasmid

#190961

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP3-E67A_rpoA-U

Plasmid

#190963

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP4_rpoA-U

Plasmid

#190964

Purpose

Test the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP4

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6-E67A_rpoA-C

Plasmid

#190972

Purpose

Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP5_rpoA-U

Plasmid

#190965

Purpose

Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP5

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FnRPR9-10

Plasmid

#182568

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FnRPR8-10

Plasmid

#182570

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Melissa Jurica, Tara Davis

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Use

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FAK C-term

Plasmid

#186147

Purpose

For expresseion of recombinant FAK c-terminal domain in E. coli

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Use

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NmMetNI-WT

Plasmid

#172114

Purpose

Expression of MetN and MetI membrane proteins

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Inserts

MetN
MetI

Use

Tags

10x His Tag and Enterokinase

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH858

Plasmid

#172176

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH859

Plasmid

#172177

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH768v2

Plasmid

#172173

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH769v2

Plasmid

#172174

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pThuAsol

Plasmid

#171675

Purpose

pThuAsol expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

SUMO-TEV-ThuAsol

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Ec-2

Plasmid

#163128

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2082

Plasmid

#155162

Purpose

Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaFΔ[248-269])-(6xHis-TEV-MlaB)

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 248-269 in MlaF

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
4E1H.95_LUMABS

Plasmid

#155208

Purpose

Expresses 4E1H.95_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95_LUMABS

Use

Tags

6x His Tag

Expression

Mutation

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET sfGFP(150TAG)

Plasmid

#133455

Purpose

expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

Depositor

Yu-Hsuan Tsai

Article

Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

Insert

sfGFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS_PROSS

Plasmid

#111645

Purpose

PROSS stabilized ACS

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_PROSS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS_4

Plasmid

#111646

Purpose

Funclib design ACS_4

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_4

Use

Tags

6xHis

Expression

Bacterial

Mutation

see depositor comments below

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS_9

Plasmid

#111647

Purpose

Funclib design ACS_9

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTac

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS_10

Plasmid

#111648

Purpose

Funclib design ACS_10

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_10

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS_14

Plasmid

#111649

Purpose

Funclib design ACS_14

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_14

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS_16

Plasmid

#111650

Purpose

Funclib design ACS_16

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_16

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge