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H446 EF1a RA-ABE6.3 p2A Puro

Plasmid

#139104

Purpose

Lentiviral expression of ABE6.3.

Depositor

Wen Xue

Article

Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

Insert

ABE RA6.3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-L107F

Plasmid

#81180

Purpose

Retroviral vector for expression of kinase active PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Expression

Mutation

L107F

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras G12V E37G

Plasmid

#12275

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pCDH-puro/Flag PD-L1 N35/3NQ

Plasmid

#121455

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N192Q, N200Q and N219Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

Plasmid

#171801

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-FLAG-hgp100-T2A-PuroR

Use

Gene tagging

Tags

Expression

Mutation

PuroR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Cenp-B-targeted Aurora B FRET sensor

Plasmid

#45232

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CENP-B targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA Oct4A -158

Plasmid

#50922

Purpose

U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Oct4A -158 sgRNA (POU5F1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPER retro puro macroH2A1 shRNA

Plasmid

#30517

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6. ():.

Insert

mH2A1 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND2-Puro

Plasmid

#172628

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

Plasmid

#163179

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-MAPKAP1.1-FHH-IRES-Puro

Plasmid

#120705

Purpose

Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 Isoform 1

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA

Plasmid

#162109

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to StrepTagII-ProtC-HA

Use

Lentiviral

Tags

StrepTagII-ProtC-HA

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-coemiRFP670-PGK-Puro

Plasmid

#186168

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

emiRFP670

Use

Marmoset targeting

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-miR-10b sponge

Plasmid

#25043

Depositor

Bob Weinberg

Article

Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

Insert

miR-10b sponge

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Use

Tags

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pSUPER retro puro macroH2A2 shRNA

Plasmid

#30518

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6. ():.

Insert

mH2A2 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 N192Q/N200Q

Plasmid

#121453

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N192Q/N219Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 EGFP pBabe puro

Plasmid

#13605

Depositor

Dean Sheppard

Insert

integrin alpha 9 (ITGA9 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only
pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

Plasmid

#161587

Purpose

expression of Myc-tagged mouse Lrrk2 without GTPase domain

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Use

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

deletion: AA1023-1879

Promoter

CMV

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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