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Lentiviral CaMKKbeta
Plasmid#33322DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationPromoterhuman PGKAvailable sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_mEGFP-GluA1c
Plasmid#114136PurposeMammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.DepositorInsertmEGFP tagged GluA1 C-terminus
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab7
Plasmid#174457PurposeExpress EGFP with late endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with late endosome-targeting sequence
UseTagssmall GTPase (Rab7)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2K930R-VSV
Plasmid#139346PurposeExpression of kinase-dead TYK2-K930R proteinDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-K930RPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
TF-bio-His
Plasmid#52184PurposeExpresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTF (TF Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-mCherry_puromycin
Plasmid#65285PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP
Plasmid#162033PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I
Plasmid#15679DepositorInsertPHAS-I (Eif4ebp1 Rat)
UseTags6xHisExpressionBacterialMutationNucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…PromoterAvailable sinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP
Plasmid#162032PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorUseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-NL1F35
Plasmid#190830PurposeFor mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-MCPIP1(delta ZF)
Plasmid#90110PurposeMammalian expression of Flag-MCPIP1(delta ZF)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationK317G, S318GPromoterCMVAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K29,30A)-mVenus
Plasmid#168485PurposeMammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB8F+12
Plasmid#190854PurposeFor mammalian expression of 3JB8F+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB8F_T1+12bp (A Dual-Specificity aptamer targeting OGT and β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbdCas9S(-10AC12)_Psyn-sgRNArodA
Plasmid#149661Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC12), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ME2/pBABE-puro
Plasmid#49164PurposeFor expression of Malic Enzyme 2 in mammalian cells from retroviral vectorDepositorInsertMalic Enzyme 2 (ME2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-shS6K1/2
Plasmid#81088Purposeexpresses an shRNA targeting both S6K1 and S6K2 (mouse)DepositorInsertS6K1 & S6K2 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
