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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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ATF4 6: mCD36.GFP

Plasmid

#21853

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

mCD36 (Cd36 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Only the first 13 AA of CD36 are fused to GFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-ELL pBacPAK8

Plasmid

#15322

Depositor

Ronald Conaway, Joan Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-TSC2-NLS

Plasmid

#192441

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2-NLS (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFLEX-m-cyclin D1 -T286A mutant

Plasmid

#86792

Purpose

mammalian expression of cyclin D1 T286A mutant

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D1 (Ccnd1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T286A

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT005

Plasmid

#182715

Purpose

Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIS1-Eef25UTR-TOPmut-renilla

Plasmid

#38236

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The first nucleotides of the 5' UTR were cha…

Promoter

Endogenous Eef2

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
DAXX-myc-his

Plasmid

#1852

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
PLG-bio-His

Plasmid

#53427

Purpose

Expresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PLG (PLG Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4A-2K-NS4B

Plasmid

#120828

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A-2K-NS4B ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

meg-3

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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NID1-bio-His

Plasmid

#52068

Purpose

Expresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NID1 (NID1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ST-SBP-mCherry

Plasmid

#65269

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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∆DUSP6

Plasmid

#27977

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

C293S: mutation in the phosphatase domain

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-mCherry

Plasmid

#65283

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-EGFP_puromycin

Plasmid

#65284

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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