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HA-doraWT_rescue_construct
Plasmid#190608PurposePuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_1
Plasmid#190700PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 1
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_2
Plasmid#190701PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 2
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_3
Plasmid#190702PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 3
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_dPC_2xChromo
Plasmid#179398PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromoDepositorInsertdPC_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q
Plasmid#147338PurposeInsect Expression of DmDCP1-GSSGmut-dsRNAresDepositorInsertDmDCP1-GSSGmut-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-DmDCP1_1-127_N
Plasmid#147050PurposeBacterial Expression of DmDCP1_1-127DepositorInsertDmDCP1_1-127 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP1-Y34A_P
Plasmid#147214PurposeInsect Expression of DmDCP1-Y34ADepositorInsertDmDCP1-Y34A (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G
Plasmid#146403PurposeBacterial Expression of DmDCP1_328-366-mut1DepositorInsertDmDCP1_328-366-mut1 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G
Plasmid#146404PurposeBacterial Expression of DmDCP1_328-366-mut2DepositorInsertDmDCP1_328-366-mut2 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G
Plasmid#146405PurposeBacterial Expression of DmDCP1_328-366-mut3DepositorInsertDmDCP1_328-366-mut3 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G
Plasmid#146406PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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1056H
Plasmid#183135PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056J
Plasmid#183136PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056K
Plasmid#183137PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-L36AL39A_T
Plasmid#147604PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
UseTagsExpressionInsectMutationTwo non silent mutations (H628R and N659S) compar…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorInsertDmCup-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationY342A, L347A, I350L, P418S, K1045NPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only
