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Showing: 2441 - 2460 of 5103 results

  3. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

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  7. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

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  9. pMS_375

    Plasmid
    #182132
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182132-plasmid-map-sequence-id-355786

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  16. pYL236

    Plasmid
    #173184
    Purpose
    CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene
    Depositor
    Thomas Cech
    Article
    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:
    Insert
    EZH2 (EZH2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173184-plasmid-map-sequence-id-341220

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  18. Bxb1_AJMA

    Plasmid
    #183760
    Purpose
    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003
    Inserts
    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    Use
    Tags
    Lck and NES
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183760-plasmid-map-sequence-id-363127

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  19. FOR human NANOS3 TALEN

    Plasmid
    #59722
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. REV human NANOS3 TALEN

    Plasmid
    #59723
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2441 - 2460 of 5103 results