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mCardinal-Rab4a-7

Plasmid

#56174

Purpose

Localization: Ras GTPase, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Rab4a (RAB4A Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-H3-23

Plasmid

#56163

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H3 (H3C2 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN2 wt-pBabe-Puro

Plasmid

#22704

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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mCardinal-Vimentin-7

Plasmid

#56178

Purpose

Localization: Intermediate Filaments, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Vimentin (VIM Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMCSF-R(mA)-luc

Plasmid

#12421

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

C/EBP binding site mutation (created a KpnI site …

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
mCardinal-Alpha-Actinin-19

Plasmid

#56152

Purpose

Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLINK cyclin T1 (1-280) myc (all 200) (P#575)

Plasmid

#14610

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) (all 200) (CCNT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

C198A, C200A, H202A, C205A. Active in 3T3 cells.

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mCardinal-CENPB-N-22

Plasmid

#56155

Purpose

Localization: Nucleus/Centromeres, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

NM_001810.5 (CENPB Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75233

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E

Plasmid

#224580

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E (E SARS-CoV-2)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E GGG

Plasmid

#224582

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E GGG (E SARS-CoV-2)

Use

Tags

GFP

Expression

Mammalian

Mutation

PBM (LLV) mutated to GGG

Promoter

CMV

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E stop

Plasmid

#224581

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 lacking its PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E stop (E SARS-CoV-2)

Use

Tags

GFP

Expression

Mammalian

Mutation

stop codon before PBM

Promoter

CMV

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast 3xFlag-SPNS1(R76A)

Plasmid

#198570

Purpose

SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

changed arginine R76 to alanine (R76A)

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1(E164K)

Plasmid

#198572

Purpose

SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

changed glutamic acid E164 to lysine (E164K)

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A mLIRΔWIR

Plasmid

#215508

Purpose

Expresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-1899)

Plasmid

#202514

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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