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Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCN DSB Repair Reporter (DRR)
Plasmid#98895PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.DepositorInsertNeomycin-T2A-ISce1-ISce1-eGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304-DCK*-IRES-GFP
Plasmid#176297PurposeExpresses DCK* and GFP from the same transcriptDepositorInsertMutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
UseLentiviralTagsV5ExpressionMammalianMutationS74E R104M D133APromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROD1_P2A_Hygro_Barcode
Plasmid#120466PurposeBarcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROD1 (NEUROD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIP
Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertring finger protein 31 (RNF31 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFLAG UBR1 SBX
Plasmid#24506DepositorInsertUBR1 (UBR1 Budding Yeast)
UseTagsFLAGExpressionYeastMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2
Plasmid#116734PurposeLentiviral expression of ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
K44A HA-dynamin 2 pcDNA3.1
Plasmid#34685DepositorInsertdynamin 2ba
UseTagsHA (variant)ExpressionMammalianMutationK44APromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
hGli3 6x his
Plasmid#84919Purposemammalian expression of Gli3DepositorInsertGli3 (GLI3 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-1
Plasmid#208790PurposeInducibly expresses human Caspase1, fused to light-activatable Cry2DepositorInsertCaspase1 (CASP1 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB-TAG-NICD
Plasmid#130934PurposePiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cellsDepositorInsertNICD (NOTCH1 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tcf/Lef:H2B-GFP
Plasmid#32610DepositorInsertTcf/Lef:H2B-GFP
UseTagsExpressionMutationPromoterhsp68Available sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt-scarRef
Plasmid#221150PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)DepositorInsertscdGreen2.1
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2.1)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-α-chain
Plasmid#133730PurposeExpresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorInsertsa-Chain_gePSI
ZsGreen1
UseTagsExpressionMammalianMutationOnly one part of the gePSI-system (aa1-141 + stop…PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1a-MG
Plasmid#175504PurposeAll-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elementsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1aAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Synapsin-cyto-iATPSnFR1.0
Plasmid#102556Purposeexpresses cytoplasmic ATP sensor in neuronsDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only