Showing: 2441 - 2460 of 8209 results
-
Plasmid#87425PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTBDepositorInsertACTB Homology Arms with mEGFP-linker (ACTB Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-7:SEC61B-mEGFP
Plasmid#87426PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61BDepositorInsertSEC61B Homology Arms with mEGFP-linker (SEC61B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertBE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
UseTagsHis6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertHF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
UseTagsHis6ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-HF-BE3
Plasmid#87439PurposeExpresses HF-BE3 with C-terminal NLS in mammalian cellsDepositorInsertHF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Tau (P301L)-VC
Plasmid#87633Purposeexpresses hTau fused to the C-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVTagsExpressionMutationPromoterU6 and cTnTAvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-I-PhoCl-HCVp
Plasmid#87697PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cellsDepositorInsertI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
UseTagsEBFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase
Plasmid#87741PurposeIPTG-inducible expression of T4 DNA ligase for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHisExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pCA24N-p50-ligase
Plasmid#87742PurposeIPTG-inducible expression of p50-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and human p50/NF-kB (amino acids 40-366)ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pACBSR-hyg
Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterblaAvailabilityAcademic Institutions and Nonprofits only -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterAvailabilityAcademic Institutions and Nonprofits only -
TLCV2-RB1
Plasmid#87836PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.DepositorInsertsgRB1 (RB1 Human)
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsExpressionMammalianMutationPromoterTight TRE promoter and U6AvailabilityAcademic Institutions and Nonprofits only -
pFC330
Plasmid#87842PurposeAMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection markerDepositorInsertsCas9
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…AvailabilityAcademic Institutions and Nonprofits only -
pFC332
Plasmid#87845PurposeAMA1 plasmid with Aspergillus optimized Cas9 and hph selection markerDepositorInsertsCas9
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 2441 - 2460 of 8209 results