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BPK1169: CAG-DmrC-NLS-3xFLAG-P65

Plasmid

#104564

Purpose

Mammalian expression vector for DmrC fused to p65 activation domain

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-p65

Use

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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TALEN1297

Plasmid

#32279

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting hey2 (hey2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-SDHAF3

Plasmid

#136013

Purpose

SDHAF3 3'UTR (NM_020186.2) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SDHAF3 3'UTR (ACN9 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-MPHOSPH8 Chromo

Plasmid

#59694

Purpose

Contains histone modification interacting domain (MPHOSPH8 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

MPHOSPH8 Chromo (MPHOSPH8 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Use

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-bArrestin2-TevC-P2A-TdTomato

Plasmid

#89869

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

R173H in TdTomato (please see depositor comments …

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDC184

Plasmid

#70055

Purpose

Co-expresses Syt1-SNARE37aa-linker complex with p045

Depositor

Axel Brunger

Article

Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

Inserts

His tagged Synaptobrevin 28-89
Syntaxin 191-256

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-His-MS2BP-G3BP1-WT

Plasmid

#136008

Purpose

WT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

MS2BP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL705

Plasmid

#140375

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-5BP-tetO-3WJdB-T

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 SCR

Plasmid

#16267

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SCR Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pJBL704

Plasmid

#140374

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-4BP-tetO-3WJdB-T

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89872

Purpose

expresses CB1R-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

CB1R-V2tail-TevN-BLITz1-TetR-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Ptdh3-CAD-Ttdh1

Plasmid

#127727

Purpose

Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ptdh3

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERTS585P-myc

Plasmid

#107498

Purpose

Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Use

Tags

Expression

Mammalian

Mutation

S585P

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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Desmin/pAS2-1

Plasmid

#97407

Purpose

Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

Desmin (DES Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC28-BSA4-G-S18

Plasmid

#127825

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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