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pAAV-hSynapsin-FLEX-soCoChR-GFP

Plasmid

#107712

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-FLPo-T2A-GFP

Plasmid

#161766

Purpose

express FLPo and GFP under the control of human synapsin promoter

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Inserts

FLPo
T2AEGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL

Plasmid

#218759

Purpose

Provides AND intersectional (Cre+Flp) expression of HA-hM4Di

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

hM4Di

Use

AAV

Tags

HA

Expression

Mutation

Promoter

hSyn

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

Plasmid

#198516

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

Plasmid

#50472

Purpose

Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter

Depositor

Bryan Roth

Has Service

AAV5

Insert

rM3D-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGW03 AAV-TBG-Cre-sgRNA

Plasmid

#192163

Purpose

AAV-TBG-Cre-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-iC++-EYFP

Plasmid

#137155

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP713-P2A-GFP

Plasmid

#197163

Purpose

Expresses the protein of miRFP713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-LSL-nlsGFP-shRNA

Plasmid

#175584

Purpose

Conditional express shRNA with control of LoxP

Depositor

Christian Wolfrum

Article

Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

Type

Empty backbone

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-WPRE

Plasmid

#130992

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

Plasmid

#201684

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1 (V106W)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA

Plasmid

#185715

Purpose

AAV expression of GFP and human α-Synuclein from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

Plasmid

#65418

Purpose

AAV packaging vector for CamKIIa driven KOR DREADD expression

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Insert

CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Expression

Mutation

Promoter

CaMKIIa

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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SLF155: AAV-syn-DO-H2B-jRGECO1a

Plasmid

#112278

Purpose

For AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switch

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-iC++-EYFP

Plasmid

#137157

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ConVERGD-eGFP-WPRE

Plasmid

#218747

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

nEF

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ConVERGD-eGFP-W3SL

Plasmid

#218746

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

nEF

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#133786

Purpose

Cre-dependent tdTomato fluorescent reporter for cell labeling

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

pAAV-EF1a-DIO-tdTomato-WPRE

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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