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Plasmid#169622PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2317
Plasmid#169624PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
C5m
Plasmid#120957PurposeMoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).DepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAM44
Plasmid#86484PurposeExpresses cbbL-mRFP1DepositorInsertcbbL-mRFP1
UseTagsmRFP1ExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM45
Plasmid#86485PurposeExpresses csoS1A-mRFP1DepositorInsertcsoS1A-mRFP1
UseTagsmRFP1ExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM69
Plasmid#86486PurposeExpresses pduA-mRFP1DepositorInsertpduA-mRFP1
UseTagsmRFP1ExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM70
Plasmid#86487PurposeExpresses eutM-mRFP1DepositorInserteutM-mRFP1
UseTagsmRFP1ExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM72
Plasmid#86488PurposeExpresses T4_gp23-mRFP1DepositorInsertT4_gp23-mRFP1
UseTagsmRFP1ExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM10
Plasmid#86483PurposeExpresses p12 and p9-mRFP1DepositorInsertp12, p9-mRFP1
UseTagsmRFP1ExpressionBacterialMutationPromoterpBADAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-Switch
Plasmid#99733PurposeFlp-based FLEX reporter (from RFP to GFP)DepositorInsertTagRFP and EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3428
Plasmid#144904PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTRIM27 (TRIM27 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVA2
Plasmid#119951PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
UseTagsExpressionBacterialMutationA110GPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180477PurposeInducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInserts5x QUAS-ZsGreen-P2A
mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh1
Plasmid#168986PurposeExpresses RFP cDNA and UBC9 shRNA 1 in mammalian cellsDepositorInsertUbiquitin Conjugating Enzyme 9 (UBE2I )
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIM700
Plasmid#74639Purposealias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only