-
Plasmid#177499PurposeMammalian Expression Plasmid of anti-HCN3 (Mouse). Derived from hybridoma N141/28.DepositorInsertanti-HCN3 (Mus musculus) recombinant mouse monoclonal antibody (Hcn3 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC446 - pAAV EF1a hPREP(S554A)
Plasmid#59968PurposeAn AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationS554APromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_NUP214:1-2090
Plasmid#211294PurposeMammalian expression of full-length human NUP214. C-terminal HiBiT tag.DepositorInsertNUP214:M1-S2090 (NUP214 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationP574S natural variant (VAR_045691). S1872N annot…PromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK8b_004
Plasmid#123892PurposeEncodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK systemDepositorInsert5' HIV LTR (3rd gen.)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CbS-Flag-TRRAP
Plasmid#32103DepositorInsertTransformation/transcription domain-associated protein (TRRAP Human)
UseTagsFlag 1xExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat6-SH2
Plasmid#46517DepositorInsertStat6 (STAT6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 510-654PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRW-CRY2olig-VHH(GFP)
Plasmid#126023Purposeexpress RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsDepositorInsertCRY2 PHRolig-VHH(GFP)
UseTagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1-HA
Plasmid#24399DepositorInsertBeclin1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat
Plasmid#169128PurposeAAV plasmid containing a mKate2 and a truncated PDE4D3DepositorInsertmKate2-PDE4DCatL
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationtruncated, Catalatic domain onlyPromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
spNW30
Plasmid#173485PurposeExpresses spNW30 in Bl21DepositorInsertspNW30
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec23A
Plasmid#166894Purposemammalian expression of Sec23A tagged with mCherryDepositorInsertSEC23A (SEC23A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-170
Plasmid#22292DepositorInsertSynaptojanin 1_170 (SYNJ1 Human)
UseTagsFlagExpressionBacterial and MammalianMutationK334R compared to Genbank ID NM_003895PromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorInsertSIRT3 (SIRT3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
C071
Plasmid#174343PurposeTemplate for making Nluc mRNA reporter with pA29.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C093
Plasmid#174344PurposeTemplate for making Nluc mRNA reporter with pA63.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C098
Plasmid#174345PurposeTemplate for making Nluc mRNA reporter with pA139.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP:FRB-FBAR
Plasmid#223620PurposeTransient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cellsDepositorInsertFBP17 (FNBP1 Human)
UseTagsFKBP, TagBFP and mGFP, FRBExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
OpenCRISPR-1
Plasmid#221565PurposeExpress OpenCRISPR-1 nuclease in human cells using a CMV promoterDepositorInsertOpenCRISPR-1
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC
Plasmid#221365PurposeMammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.DepositorInsertAnti-Rho IPI-[1D4] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
Plasmid#221366PurposeMammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.DepositorInsertAnti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-2xMARS-nGFP-puro
Plasmid#205238PurposeLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobodyDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseLentiviralTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcdna 3.1 DSG-3 Tension Sensor
Plasmid#182717Purposeexpresses DSG-3 TSmodDepositorInsertdesmoglein-3 (Dsg3 Mouse)
UseTagsExpressionMammalianMutationTSmod inserted between G726 and G727PromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-PuroR-Z
Plasmid#218904Purposegolden gate cloningDepositorInserthPGK-PuroR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-SunTag-RPS7-PP7-WPRE
Plasmid#198338PurposePB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loopsDepositorInsertGCN4x24-RPS7-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loops and SunTagExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-HCN4 [N114/10R-2b]
Plasmid#206653PurposeMammalian Expression Plasmid of anti-HCN4 (Human). Derived from hybridoma N114/10-2b.DepositorInsertanti-HCN4 (Homo sapiens) recombinant Mouse monoclonal antibody (HCN4 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-V5
Plasmid#214985PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tagDepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HDR-HaloPOLQ
Plasmid#211637Purposehalo donorDepositorInsertPuro-LoxHaloTag
UseTagsflagExpressionMammalianMutationPromoterSV40Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
KOR untagged
Plasmid#208918Purposeencodes the human kappa opioid receptor, without any tagsDepositorInsertOPRK1 (OPRK1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3061
Plasmid#144537PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSNAPC3 (SNAPC3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ECT2_sgRNA
Plasmid#183873PurposepX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorInsertECT2 sgRNA spacer (ECT2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCN4 scFv [N114/10]
Plasmid#190526PurposeMammalian Expression of HCN4 scFV. Derived from hybridoma N114/10.DepositorInsertHCN4 (Homo sapiens) recombinant scFV (HCN4 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-TMPRSS2-A6
Plasmid#188690PurposesgRNADepositorInsertsgTMPRSS2 (TMPRSS2 Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-ECT2
Plasmid#183835PurposeRepair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertECT2 homology arms with mNeonGreen-linker (ECT2 Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-134/150TAG
Plasmid#174077PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationD134TAG + N150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKmyc-Borg3
Plasmid#141074PurposeExpresses Borg3 (Cdc42ep5) in mammalian cellsDepositorInsertBorg3 (Cdc42ep5 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK7_014
Plasmid#123881PurposeEncodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK systemDepositorInsert3' HIV LTR (3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_031
Plasmid#123809PurposeEncodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK systemDepositorInsertKRAB:meCP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
BSP606
Plasmid#122251Purposeexpression vector for mECitrine under EFT3 promoterDepositorInsertmECitrine
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD01 S. cerevisiae telomerase RNA KBS
Plasmid#61890PurposePlasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV p16 INK4A
Plasmid#10916DepositorInsertp16 INK4 (CDKN2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-Puro
Plasmid#79049PurposeExpresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf1-16
Plasmid#198271PurposeUnmodified pPVC; contains WT PVC structural/accessory genesDepositorInsertPVCpnf1-16
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Abl-His6-FLAG
Plasmid#52684Purposeexpression of Abl in mammalian cellsDepositorInsertABL1 (Abl1 Mouse)
UseTagsFLAG and His6ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF Synaptophysin-GCamp6s
Plasmid#188981PurposePresynaptic fluorescent Ca2+ indicatorDepositorInsertSynaptophysin (Syp Synthetic, Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL39
Plasmid#15458DepositorInsert
UseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2m
Plasmid#140553PurposeExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2m (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only