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Plasmid#53745PurposeContains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsmRFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
UseTagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of FireFly luciferase stop codon between the ORF and MCS and polyADepositorInsertFireFly luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.eGFP.WPRE.bGH
Plasmid#105535PurposeAAV expression of EGFP from TBG promoterDepositorHas ServiceAAV8InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterTBGAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8r
Plasmid#208294PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ITSN1
Plasmid#129614PurposeRhoGEFDepositorInsertITSN1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_P2A_GFP
Plasmid#112099PurposeC:G-to-T:A base editingDepositorInsertBE4max_P2A_GFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-300(HAT-)
Plasmid#23254DepositorInsertp300 (EP300 Human)
UseTagsExpressionMammalianMutationH1415A E1423A Y1424A L1428S Y1430A H1434APromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CDK4
Plasmid#116724PurposeLentiviral expression of CDK4DepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX2-Tango
Plasmid#66440PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMRGPRX2 (MRGPRX2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MetGFP
Plasmid#37560DepositorInsertMET (MET Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAKgfplux1
Plasmid#14083DepositorInsertBacterial luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF
Plasmid#82021PurposecLac143; pALM143; IPTG inducible systemDepositorInsertYFP
UseTagsExpressionMutationPromoterPcptoo_C38T,A39TAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Hgpm2
Plasmid#164441PurposeEncodes codon-optimized HIV gagpol for pseudovirus productionDepositorInsertCodon Optimized HIV-1 GagPol
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FlucDM-HA-GFP11-N1
Plasmid#91956PurposeExpresses aggregation-prone FlucDM-HA-GFP11 in mammalian cellsDepositorInsertFlucDM-HA-GFP11
UseTagsExpressionMammalianMutationR188Q,R261QPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 ORF7A
Plasmid#141276PurposeGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TetO-Ascl1-puro
Plasmid#97329PurposeGenerating lentiviruses to express Ascl1 and puromycin resistanceDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTET-ONAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Blast
Plasmid#199622PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C3)-Nup153
Plasmid#64268PurposeGFP-tagged mammalian expression of Nup153DepositorInsertNuP153 (NUP153 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3G-LSD1-dCas9-P2A-mCherry
Plasmid#138462Purpose2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherryDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP-ICP0-C3
Plasmid#134559PurposeExpresses YFP-ICP0 fusion proteinDepositorInsertICP0
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM51323
Plasmid#153224PurposeLevel 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminatorDepositorInsertArabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPCXU6PT7SK
Plasmid#27549DepositorInsertU6PT7SK (RN7SK Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST
Plasmid#119749PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.DepositorTypeEmpty backboneUseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CASR-Tango
Plasmid#66235PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCASR (CASR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTF102
Plasmid#134774PurposeA binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dSpRY-Act3.0
Plasmid#158416PurposeCRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMito.iGABASnFR
Plasmid#112175PurposeGABA SensorDepositorInsertiGABASnFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-PP7-2xmCherry-CAAX
Plasmid#74925PurposeExpresses PP7-2xmCherry-CAAXDepositorInsertPP7-2xmCherry-CAAX
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE4
Plasmid#87087PurposeExpresses ApoE4 in Mammalian cellsDepositorInsertApolipoprotein E4 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
PRMT8-FLAG
Plasmid#164699PurposeExpression of PRMT8 with a C-terminal FLAG tagDepositorInsertPRMT8 (PRMT8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRmRuby2
Plasmid#59148PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only