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Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only
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pCAG-iCre
Plasmid#89573PurposeExpresses iCre under pCAGDepositorInsertiCre
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9 plasmid
Plasmid#100091PurposedCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expressionDepositorTypeEmpty backboneUseCRISPRTags3XFlag-NLS-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-loxp Kir2.1-2A-GFP
Plasmid#161574PurposeExpression of Kir2.1-2A-GFP in a Cre-dependent fashionDepositorInsertKir2.1-2A-GFP (Kcnj2 Mouse)
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-NpHR-EYFP
Plasmid#171621PurposeTRE-NpHR-EYFP reporterDepositorInsertTRE-NpHR-EYFP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
Plasmid#203348PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertEnhanced Green Fluorescent Protein
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST ERKKTRmRuby2
Plasmid#90231PurposeLentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRVL-4
Plasmid#104582PurposeContains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.DepositorInsertHuman CL kappa
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40
Plasmid#105551PurposeAAV expression of GFP-Cre from CamKII promoterDepositorHas ServiceAAV Retrograde and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-PSMA
Plasmid#11524DepositorInsertprostate-specific membrane antigen (FOLH1 Human)
UseTagsHisExpressionInsectMutationThere is a short N-terminal truncation of this pr…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-STAT3
Plasmid#111934PurposeExpresses human STAT3 fused to GFP in mammalian cellsDepositorInsertSignal transducer and activator of transcription 3 (STAT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-3
Plasmid#27289DepositorInsertintegrin beta 3 (ITGB3 Human)
UseTagsHis and MycExpressionMammalianMutationn/aPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.eGFP.WPRE.hGH
Plasmid#105549PurposeAAV expression of EGFP from GFAP promoterDepositorHas ServiceAAV5InserteGFP
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only