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Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)
Plasmid#180587PurposeFdCdDepositorInsertCre
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP-G3BP2(1)
Plasmid#136060PurposeG3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)DepositorInsertG3BP2 (G3BP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-PuroR Xlone eGFP
Plasmid#179837PurposeDoxycycline-inducible expression of eGFPDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALEN ; Donor plas…TagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TM-CIBN-BLITz1-TetR-VP16
Plasmid#89878Purposeexpresses TM-CIBN-BLITz1-TetR-VP16 of BLITz systemDepositorInsertTM-CIBN-TevN-BLITz1-TetR-VP16
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
UseTagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-CRY2PHR-TevC
Plasmid#89877Purposeexpresses NES-CRY2PHR-TevC of BLITz systemDepositorInsertNES-CRY2PHR-TevC
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCH68 (dAsCas12a-3xKRAB piggyBac)
Plasmid#217334PurposepiggyBac expression of dAsCas12a-3xKRABDepositorInsertdAsCas12a
UseCRISPRTagsnucleoNLS-3xKRAB-3xHA-P2A-TagBFP2ExpressionMammalianMutationE993APromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro-{beta}4 siRNA
Plasmid#16266DepositorInsertSIRNA Beta 4 Integrin (ITGB4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4RFP
Plasmid#52489PurposeExpresses TagRFP-TDepositorInsertTagRFP-T
UseRabies virusTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
Plasmid#46676DepositorInserthsa-mir-133a-1 (MIR133A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP-G3BP2(2)
Plasmid#136061PurposeG3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)DepositorInsertG3BP2 (G3BP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-rCL1-HormR-GAIN
Plasmid#37847DepositorInsertCL1 (Adgrl1 Rat)
UseBaculovirus transfer vectorsTags8xHISExpressionMutationHormR and GAIN domains only (aa460–849PromoterPolyhedrinAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-L11
Plasmid#127830PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1 R44Q-14-mVenusC
Plasmid#191394PurposeR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1 R44Q-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationR44QPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only