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Plasmid#173131PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj4 (kcnj4 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj9
Plasmid#173135PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj9 (kcnj9 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11l
Plasmid#173140PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11l (kcnj11l Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-LMTK2_IDG-K
Plasmid#170580PurposeGateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-LMTK2 (LMTK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-EEF2K_IDG-K
Plasmid#170592PurposeGateway destination clone of EEF2K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-EEF2K (EEF2K Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-HIPK3-V5-miniTurbo_IDG-K
Plasmid#158191PurposeGateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertHIPK3-V5-miniTurbo (HIPK3 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-U6-BsmBI-R5
Plasmid#141032PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR-P3P1r
Plasmid#141014PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R2-pA-L4
Plasmid#141062PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
CSIV-DEST
Plasmid#141053PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R1R4
Plasmid#141054PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R5R4
Plasmid#141055PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4-H1-BsmBI-L2
Plasmid#141034PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4-H1-BbsI-L2
Plasmid#141033PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-U6-BbsI-R5
Plasmid#141031PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R2L4
Plasmid#141061PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF8_isoD_CRISPR_resistant
Plasmid#122468PurposepDONR for Gateway cloningDepositorInsertSCAF8 isoform D CRISPR resistant (SCAF8 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF4_CRISPR resistant
Plasmid#122465PurposepDONR for Gateway cloningDepositorInsertSCAF4 CRISPR resistant (SCAF4 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 no-stop
Plasmid#123207PurposeGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only