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LR (hsp70I: cPLA2-mK2)

Plasmid

#162564

Purpose

Measuring cPLA2 activation in live cells after heat shock.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

Heat shock protein 70 (HSPA1A Human)
cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Gateway donor vector

Tags

mK2

Expression

Mutation

Promoter

cmlc2

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFETCh_HMG20A

Plasmid

#86271

Purpose

Donor vector for 3' FLAG tag of human HMG20A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HMG20A homology arms (HMG20A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_KLF11

Plasmid

#86269

Purpose

Donor vector for 3' FLAG tag of human KLF11

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF11 homology arms (KLF11 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00403

Plasmid

#34513

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8013 (Su(z)12 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00211

Plasmid

#34362

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4491 (noc Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00246

Plasmid

#34381

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2711 (dwg Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00254

Plasmid

#34386

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5575 (ken Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00164

Plasmid

#34317

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4354 (slbo Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00181

Plasmid

#34334

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5393 (apt Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCRISPR-HOT-P2A-Clover-BlastR

Plasmid

#138568

Purpose

Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

P2A-clover-BlastR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

CAG, EF-1alpha, and TRE3G

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

Plasmid

#193465

Purpose

Dn29 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Dn29_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-JNKAR

Plasmid

#181857

Purpose

Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-JNKAR

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Use

Tags

2A-EGFP

Expression

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-RHOA

Plasmid

#183836

Purpose

Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA homology arms with mNeonGreen-linker (RHOA Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-mScarlet-I-CD4-bla

Plasmid

#189980

Purpose

Donor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

mScarlet-I

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDsRed-attP

Plasmid

#51019

Purpose

Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

Depositor

Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Inserts

attP
LoxP-3xP3-DsRed-LoxP

Use

High copy, amp resistance

Tags

Expression

Mutation

Promoter

3xP3 and None

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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