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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mFoxa2

Plasmid

#33014

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Forkhead box A2 (Foxa2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H10L

Promoter

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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PR_GEF YFP (2XPDZ-YFP-LARG DH)

Plasmid

#80408

Purpose

Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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CDK4-3XFlag-pBabe

Plasmid

#73558

Purpose

vector encoding a fusion protein between CDK4 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1(apf)

Plasmid

#13846

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(apf) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pJM31

Plasmid

#52986

Purpose

A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Annexin V (ANXA5 Human)

Use

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-LOXCATmut

Plasmid

#140085

Purpose

A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

Depositor

Vamsi Mootha

Article

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Use

Tags

His6

Expression

Bacterial

Mutation

Mutation in H265A and R268A in LOX (used original…

Promoter

T7

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-C-p50

Plasmid

#180238

Purpose

Expresses p50 in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Proc Natl Acad Sci U S A. 2016 Sep 6;113

Insert

p50 (DCTN2 Human)

Use

Tags

pmCherry-C1

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry KNL1 wt hardened

Plasmid

#45224

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)

Use

Tags

TEV S15

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
CMV-YE1-BE3-FNLS-CMV-mCherry

Plasmid

#154005

Purpose

Express YE1-BE3-FNLS

Depositor

Hui Yang

Article

Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

Insert

YE1-BE3-FNLS

Use

CRISPR

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGMKOSiE

Plasmid

#20865

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Mar 1. ():.

Insert

myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

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F2RL1-Tango

Plasmid

#66277

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

F2RL1 (F2RL1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Clathrin light chain

Plasmid

#83032

Purpose

Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Clathrin light chain (CLTA Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCK301

Plasmid

#87767

Purpose

E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Insert

PrhaBAD-sfGFP

Use

Synthetic Biology

Tags

6xHis Tag

Expression

Bacterial

Mutation

Promoter

PrhaBAD rhamnose-inducible promoter from E. coli

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 508)

Plasmid

#195030

Purpose

GAL4-Creb5 fusion construct (full length, 508aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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