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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

Plasmid

#101285

Purpose

Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-H-Ras

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H-Ras V12

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-E2F1

Plasmid

#202520

Purpose

Expressing human E2F1 protein with FLAG-tag

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

E2F1 (E2F1 Human)

Use

Tags

FLAG-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eC

Plasmid

#201903

Purpose

non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

Insert

Synthetic construct isolate AAV-PHP.eC VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-s ONE-GO

Plasmid

#204327

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Venus

Plasmid

#70267

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

Cas9
Venus Reporter

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS and P2A

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIP-mCherry-Halo-YAP1

Plasmid

#128336

Purpose

Retrovirus construct to express mCherry-Halo-YAP1

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Insert

YAP1 (YAP1 Human)

Use

Retroviral

Tags

mCherry and Halo-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

Plasmid

#132773

Purpose

Expression of human LMNA.

Depositor

Jan Lammerding

Article

Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FKBP25-V5-APEX2

Plasmid

#83418

Purpose

Expressed FKBP25/FKBP3-V5-APEX2 in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

Peptidyl-prolyl cis-trans isomerase FKBP3 (FKBP3 Human)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA1

Plasmid

#89719

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA1 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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cyclin D1-HA-pBabe-Hygro

Plasmid

#22703

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(mB)-luc

Plasmid

#12422

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

AML1 binding site mutation (created a KpnI site i…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pX335A_hCas9(D10A)_IRF8gRNA2

Plasmid

#89720

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA2 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mCardinal-H2B-C-10

Plasmid

#56162

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H2B (H2BC11 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLVX-FADD-DD

Plasmid

#58263

Purpose

Lentiviral vector for expressing a truncated form of FADD (lack of death effector domain) in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

FADD (FADD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

version of FADD (FADD-DD) that contains the FADD …

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
mCardinal-Tubulin-C-18

Plasmid

#56176

Purpose

Localization: Microtubules, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tubulin (TUBA1B Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits