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Plasmid#207319PurposeFunctional incorporation of SNAP-tag into envelope of virionsDepositorInsertSNAP-tag
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme Ī²-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is ā¦PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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opto-Itsn1
Plasmid#174509PurposeExpresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-Itsn1-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW1-Myc-Sarm1
Plasmid#50707Purposeexpression of Sarm1 in mammalian cellsDepositorInsertSarm1 (Sarm1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJR022
Plasmid#198820PurposeC. elegans optimized version of mScarletDepositorInsert15xUAS::wrmScarlet::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACBE
Plasmid#204607PurposeMammalian expression of adenine-to-cytosine base editor ACBEDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
UseTagsGSTExpressionBacterialMutationPromoterlac promoter / tac promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCT2
Plasmid#45799DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-NLS-AcrIIA4
Plasmid#113038PurposeAAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
Plasmid#173862PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)DepositorInsertAktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-sfGFP-DsRed
Plasmid#80811PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertsfGFP-piggyBac-DsRed
UseUnspecifiedTagssfGFPExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a_CnCas12f1
Plasmid#204996PurposeCnCas12f1 heterologous expression plasmidDepositorInsertCnCas12f1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Flag-IDH1
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorInsertisocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPp65ADZpUw
Plasmid#26234DepositorInsertp65AD-Zip (RELA Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5A-SH2
Plasmid#46515DepositorInsertStat5A (STAT5A Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 582-693PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO8Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PTK7
Plasmid#23943DepositorInsertPTK7 (PTK7 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PDGF/B-zeo
Plasmid#17757DepositorInsertPDGF/B (PDGFB Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AcGFP1-ORP9-PH
Plasmid#214266PurposeMammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PHDepositorArticleInsertAcGFP1-ORP9(1-111) (Osbpl9 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xCSL-luciferase
Plasmid#41726DepositorInsert4xCSL binding sites
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains a multimerized high affinity CSL bindingā¦PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PCYT2
Plasmid#81074PurposeExpresses PCYT2 in mammalian cellsDepositorInsertphosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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CGBE1 (pRZ3885)
Plasmid#140252PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorInserteUNG-BE4max(R33A)-ĪUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL046
Plasmid#198807PurposeIn vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.DepositorInsert15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0701
Plasmid#143065PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAXBP1 (PAXBP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5B-SH2
Plasmid#46516DepositorInsertStat5B (STAT5B Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 593-692PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-SauriABE8e
Plasmid#189927PurposePlasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertSauriABE8e
UseTagsExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#138149PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertCasRx
UseLentiviralTagsNLS-HAExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Blast)
Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleavā¦TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-KLC3-N-18
Plasmid#62760PurposeLocalization: Kinesin Light Chain , N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertKLC3 (Klc3 Mouse)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CamK2a(0.4)-Opto-cytTrkB(E281A)-HA
Plasmid#180589PurposeOpto-cytTrkBDepositorInsertTrkB
UseAAVTagsExpressionMutationPHR(E281A)PromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN PSAM4 GlyR IRES EGFP
Plasmid#119742PurposeChemogenetics expression plasmidDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
Plasmid#222742PurposeCaST-IRES for neuron expressionDepositorInsertCD4-sTC-M13-GFP-IRES-CaM-V5-sTN
UseTagsHA and V5ExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNTI821
Plasmid#216630PurposeTet-inducible Cal-ID (V5) for Sleeping Beauty transposon systemDepositorArticleInsertCal-ID
UseSleeping beauty transposon systemTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-pBAD
Plasmid#54622PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and mEGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV Nup62-V5
Plasmid#175116PurposeLentiviral expression of mouse Nup62-V5DepositorInsertNup62 (Nup62 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107576PurposeB. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKIā¦ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterpdh promoter Bacillus megaterium DSM319Available sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-dCherry-FRB
Plasmid#172444PurposeExpression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRBDepositorInsertCacng2 (Cacng2 Mouse)
UseTagsdCherry-HA-FRBExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK0_003
Plasmid#123925PurposeEncodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK systemDepositorInsertCas9-sgRNA destination
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo19
Plasmid#127612PurposeGFP-tagged Myosin-19DepositorInsertMyo19
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-GFPplus
Plasmid#172718PurposeExpression of a fast folding GFP variant GFP plusDepositorInsertGFP plus
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only