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Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE Tight ArchT-GFP
Plasmid#79627PurposeTetracycline-dependent expression of ArchT-EGFP in mammalian cellsDepositorInsertArchT
UseTagsEGFPExpressionMammalianMutationArchT is a more sensitive version of Arch (archae…PromoterTet responsive Ptight promoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EPB41L3_FERM
Plasmid#110011PurposeProtein expression and purification of EPB41L3_FERMDepositorInsertEPB41L3_FERM (EPB41L3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-FLAG
Plasmid#105783PurposeExpression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/BirA/FLAG-DDX54
Plasmid#97063PurposeExpresses BirA/FLAG-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsBirA and FLAGExpressionMammalianMutationPromotertetO2Available sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MAPK3_Pkinase
Plasmid#110023PurposeProtein expression and purification of MAPK3_PkinaseDepositorInsertMAPK3_Pkinase (MAPK3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AP2M1_Adap-comp-sub
Plasmid#109855PurposeProtein expression and purification of AP2M1_Adap-comp-subDepositorInsertAP2M1_Adap-comp-sub (AP2M1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T74A
Plasmid#47939Purposeexpresses Myc tagged human MOB1 containing T74A mutationDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT74APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mEGFP
Plasmid#105794PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
ET1-human LRRC33
Plasmid#111603PurposeExpress human LRRC33 in 293T cellsDepositorInserthuman LRRC33 (NRROS Human)
UseTagsPreScission/TEV/ProteinC/6xHIS/SBP/ExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDN(1-400)-GFP
Plasmid#111862PurposeC-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDN(1-400)-GFP (Bicd2 Mouse)
UseTags8xHis tag, GFP, and ZZ tagExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)
Plasmid#133793PurposeU6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017DepositorInsertCjeCas9 sgRNA entry vector
UseTagsExpressionMammalianMutationV2-TGAA linker sgRNA architecture from Kim et al.…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
His10-CD3z-SNAPf-ybbR (XSB507)
Plasmid#78508PurposeConstruct for expressing CD3z-SNAPf-ybbR in E. ColiDepositorInsertCD3z (Cytoplasmic domain) (CD247 Human)
UseTagsHis10 and SNAPf-ybbRExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mTagBFP
Plasmid#105789PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mTagBFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-FLAG-YWAA-pGK-HYG
Plasmid#107375PurposeLentiviral expression of FLAG-YWAADepositorInsertcereblon (CRBN Human)
UseLentiviralTagsFLAGExpressionMammalianMutationCRBN-Y383A/W385APromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T12A
Plasmid#47936Purposeexpresses Myc tagged human MOB1 containing T12A mutationDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT12APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiex1-SYNAC
Plasmid#82710PurposeSynthetic soluble adenylyl cyclase FRET sensor and cAMP generatorDepositorUseTagsFLAG, mCerulean, and mCitrineExpressionBacterial and InsectMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_U6_sgRNA_Mettl3C
Plasmid#165420PurposesgRNA construct for targeting Mettl3 C-terminusDepositorInsertPspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1 VAMP2 R125TAG
Plasmid#69877Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2DepositorInsertVAMP2 (Vamp2 Rat)
UseSynthetic BiologyTagsExpressionMammalianMutationAmber and Ochre stop codons in the former linker …PromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max_KKH C-terminal
Plasmid#137181PurposeExpresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoterDepositorInsertNpu-saBE3.9max_KKH_C-terminal
UseTagsExpressionMammalianMutationE782K;N968K;R1015H conferring recognition of NNNR…PromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAO1_G-alpha
Plasmid#110013PurposeProtein expression and purification of GNAO1_G-alphaDepositorInsertGNAO1_G-alpha (GNAO1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CASP2_Peptidase-C14
Plasmid#109996PurposeProtein expression and purification of CASP2_Peptidase-C14DepositorInsertCASP2_Peptidase-C14 (CASP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HLA-DRB1_MHC-II-beta
Plasmid#110021PurposeProtein expression and purification of HLA-DRB1_MHC-II-betaDepositorInsertHLA-DRB1_MHC-II-beta (HLA-DRB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC- IKZF5-3xHA-pGK-PUR
Plasmid#107385PurposeLentiviral expression of IKZF5-3xHADepositorInsertIKZF5 (IKZF5 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUBCAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA13_G-alpha
Plasmid#109913PurposeProtein expression and purification of GNA13_G-alphaDepositorInsertGNA13_G-alpha (GNA13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-SNAPf-Psc-StrepII
Plasmid#111860PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.DepositorInsertHook3(1-552)-SNAPf (HOOK3 Human)
UseTagsPsc, SNAPf, and Strep-IIExpressionInsectMutationOptimised for expression in Sf9 cellsPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESR2_Hormone-recep
Plasmid#109907PurposeProtein expression and purification of ESR2_Hormone-recepDepositorInsertESR2_Hormone-recep (ESR2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1
Plasmid#111459PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523), C643APromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAT1_G-alpha
Plasmid#109946PurposeProtein expression and purification of GNAT1_G-alphaDepositorInsertGNAT1_G-alpha (GNAT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_APLP2_Kunitz-BPTI
Plasmid#110041PurposeProtein expression and purification of APLP2_Kunitz-BPTIDepositorInsertAPLP2_Kunitz-BPTI (APLP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_aman-2::CemOrange2 (aman-2 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAZ_G-alpha
Plasmid#109949PurposeProtein expression and purification of GNAZ_G-alphaDepositorInsertGNAZ_G-alpha (GNAZ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL
Plasmid#63886PurposeAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.DepositorInsertER-localized GCaMP3(wt)
UseAAVTagsExpressionMutationPromoterhuman SYN1Available sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
PHR-LAT Y6F YFFF-mCitrine (XSB589)
Plasmid#78517PurposeLentiviral vector for expressing LAT-Y6F YFFF-mCitrine (0 Grb2 binding sites)DepositorInsertLAT (LAT Human)
UseLentiviralTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgCIC-2
Plasmid#74967PurposesgCIC-2, puromycin selectionDepositorInsertCIC (CIC Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708/710A
Plasmid#70153Purposemammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutationsDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationS708A, S710APromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 1-154 (XSB382)
Plasmid#78510PurposeConstruct for expressing Grb2 lacking the C-terminal SH3 domain in E. ColiDepositorInsertGrb2 1-154 (GRB2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FRS2_IRS
Plasmid#109815PurposeProtein expression and purification of FRS2_IRSDepositorInsertFRS2_IRS (FRS2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_L58R
Plasmid#101780PurposeExpresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationL58R mutationPromoterT5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only