Purpose
CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
Insert
human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
Use
CRISPR; In vitro transcription; t7 promoter
Tags
P2A-EGFP
Expression
Mammalian
Mutation
nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
Available since
March 26, 2020
Availability
Academic Institutions and Nonprofits only