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Plasmid#199218PurposeLPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP::BACH1 (BACH1 Human)
UseTagsEGFPExpressionMammalianMutationsilient mutation at nucleic acid 177 C changed to…PromoterCMV D2ir promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRP_8xgRNA-FLuc
Plasmid#135937PurposeFirefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.DepositorInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
UseLuciferaseTagsExpressionMammalianMutationPromoterminCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.4/5 Spike
Plasmid#186031PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4/5 SpikeDepositorInsertSARS-CoV-2 BA.4/5 Spike (S )
UseTagsExpressionMammalianMutationBA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BQ.1.1 Spike
Plasmid#193710PurposeExpresses codon-optimized full length SARS-CoV-2 BQ.1.1 SpikeDepositorInsertSARS-CoV-2 BQ.1.1 Spike (S )
UseTagsExpressionMammalianMutationContains the following mutations: T19I, LPPA24S, …Promoterchicken β-actin promoter, CMV enhancerAvailable sinceNov. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BA.2.75 Spike
Plasmid#190012PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2.75 SpikeDepositorInsertSARS-CoV-2 BA.2.75 Spike (S )
UseTagsExpressionMammalianMutationContains the BA.2 mutations (T19I, LPPA24S, G142D…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceSept. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BA.4.6 Spike
Plasmid#190013PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4.6 SpikeDepositorInsertSARS-CoV-2 BA.4.6 Spike (S )
UseTagsExpressionMammalianMutationContains the following mutations: T19I, LPPA24S, …Promoterchicken β-actin promoter, CMV enhancerAvailable sinceSept. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BA.2 Spike
Plasmid#185604PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2 SpikeDepositorInsertSARS-CoV-2 BA.2 Spike (S )
UseTagsExpressionMammalianMutationT19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJuly 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BA.1 Spike
Plasmid#185452PurposeExpresses codon-optimized full length SARS-CoV-2 BA.1 SpikeDepositorInsertSARS-CoV-2 Spike BA.1 (S )
UseTagsExpressionMammalianMutationContains the following mutations: A67V, Δ69-70, T…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits