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Plasmid#108834PurposeInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBP,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Plasmid#108835PurposeInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpA
Plasmid#108823PurposeExpresses N-terminus to aa270 of Cre recombinase fused to nMag CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA
Plasmid#108738PurposeExpresses aa271 to C-terminus of Cre recombinase fused to FKBP CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75243PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpA
Plasmid#108783PurposeExpresses N-terminus to aa172 of VCre recombinase fused to ABI CIDDepositorInsertVCre recombinase fragment N-terminus to aa172
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2587_pCAG-PV1-PYL-L1-VCre-173C-NLS-BGHpA
Plasmid#108784PurposeExpresses aa173 to C-terminus of VCre recombinase fused to PYL CIDDepositorInsertVCre recombinase fragment aa173 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA
Plasmid#108813PurposeExpresses N-terminus to aa326 of TP901 integrase fused to GID1 CIDDepositorInsertTP901 integrase fragment N-terminus to aa326
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA
Plasmid#108801PurposeExpresses N-terminus to aa428 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa428
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA
Plasmid#108765PurposeExpresses N-terminus to aa168 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa168
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA
Plasmid#108766PurposeExpresses aa169 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa169 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA
Plasmid#108727PurposeExpresses N-terminus to aa256 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA
Plasmid#108728PurposeExpresses aa257 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa257 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
Plasmid#69224PurposeExpresses R1335K mutant SpCas9 fused to Zif268 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLS and Zif268ExpressionMammalianMutationArginine 1335 is changed to LysinePromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA
Plasmid#108817PurposeExpresses N-terminus to aa229 of Cre recombinase fused to nMag CIDDepositorInsertCre recombinase fragment N-terminus to aa229
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA
Plasmid#108725PurposeExpresses N-terminus to aa251 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa251
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA
Plasmid#108815PurposeExpresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CIDDepositorInsertBxb1 integrase fragment N-terminus to aa468
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA
Plasmid#108832PurposeExpresses aa397 to C-terminus of Flp recombinase fused to pMag CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA
Plasmid#108785PurposeExpresses N-terminus to aa269 of VCre recombinase fused to ABI CIDDepositorInsertVCre recombinase fragment N-terminus to aa269
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only