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Plasmid#169624PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZFN-AAVS1-KKR
Plasmid#159298PurposeZFN (right) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertZFN targeting the AAVS1 locus
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-WT-PTPN22
Plasmid#195376PurposeMammalian expression of human wild-type PTPN22 with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfCherry11-WT-PTPN22
Plasmid#195384PurposeMammalian expression of human wild-type PTPN22 with sfCherry(11) tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagssfCherry(11)ExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfCherry11-620W-PTPN22
Plasmid#195386PurposeMammalian expression of a human PTPN22 mutant (620W) with sfCherry(11) tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagssfCherry(11)ExpressionMammalianMutationR620W (rs2476601)PromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HT7-mTurquoise2-KDEL
Plasmid#188571PurposeExpresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.DepositorInsertHaloTag7
UseTagsKDEL and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-1858T-PTPN22
Plasmid#195378PurposeMammalian expression of a human PTPN22 mutant (1858T) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationT at rs2476601 (C>T)PromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 68CVQ
Plasmid#13691DepositorInsertHPV 16 E7 CVQ6870AAA (E7 Human)
UseTagsExpressionMammalianMutationaa 68-70 changed from CVQ to AAAPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 H2P
Plasmid#13698DepositorInsertHPV 16 E7 H2P (E7 Human)
UseTagsExpressionMammalianMutationaa 2 from H to PPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 del L67
Plasmid#13703DepositorInsertHPV 16 E7 del L67 (E7 Human)
UseTagsExpressionMammalianMutationdel aa L67PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 E26G
Plasmid#13701DepositorInsertHPV 16 E7 E26G (E7 Human)
UseTagsExpressionMammalianMutationaa 26 E to GPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 L67R
Plasmid#13702DepositorInsertHPV 16 E7 L67R (E7 Human)
UseTagsExpressionMammalianMutationaa 67 L to RPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 C24G
Plasmid#13692DepositorInsertHPV 16 E7 C24G (E7 Human)
UseTagsExpressionMammalianMutationaa 24 changed from C to GPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 L67K
Plasmid#13694DepositorInsertHPV 16 E7 L67K (E7 Human)
UseTagsExpressionMammalianMutationaa 67 from L to KPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 L67E
Plasmid#13741DepositorInsertHPV 16 E7 L67E (E7 Human)
UseTagsExpressionMammalianMutationaa 67 from L to EPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 del EDE
Plasmid#13690DepositorInsertHPV 16 E7 del EDE (E7 Human)
UseTagsExpressionMammalianMutationdel aa 35-37PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 E46A
Plasmid#13693DepositorInsertHPV 16 E7 E46A (E7 Human)
UseTagsExpressionMammalianMutationaa 46 changed from E to APromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 N245
Plasmid#13695DepositorInsertHPV 16 E7 N245 (E7 Human)
UseTagsExpressionMammalianMutationH2G, D4G, T5M mutationsPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
WT-short-UBASH3A-GFP11
Plasmid#195385PurposeMammalian expression of human wild-type short-form UBASH3A with GFP(11) tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsGFP(11)ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -