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pMRE-Himar-134

Plasmid

#185925

Purpose

Himar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mOrange2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 1-2

Plasmid

#54356

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pRATIO2112

Plasmid

#141296

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Article

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Use

Tags

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBzCas13b

Plasmid

#164862

Purpose

Inducible expression of BzCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

Bergeyella zoohelcum Cas13b

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBP

Plasmid

#72947

Purpose

Backbone vector with internal BsmBI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDest-676 (attR4-attR3)

Plasmid

#161888

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-686 (attR4-attR2)

Plasmid

#161889

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LIIbeta R 3-4

Plasmid

#54361

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

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pDest-305 (attR4-attR2)

Plasmid

#161895

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRATIO2151

Plasmid

#141300

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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LIIbeta R 5-6

Plasmid

#54365

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI+Bpi

Plasmid

#54339

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLPS-hAkt1-gfp

Plasmid

#49388

Purpose

mammalian expression of human Akt1 fused to EGFP

Depositor

Randall McKinnon

Insert

Akt1 (AKT1 Human)

Use

Cre/Lox; Acceptor vector

Tags

EGFP

Expression

Mammalian

Mutation

tga to cca at nt 1725 with respect to GenBank ID …

Promoter

CMV

Available since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only
KpAvs4

Plasmid

#188796

Purpose

Avs4 from Klebsiella pneumoniae UNMC_8078

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEG3a

Plasmid

#31398

Depositor

Kenn Gerdes

Article

Ebersbach et al Mol Microbiol. 2008 May . 68(3):720-35.

Insert

zapB (zapB E. coli)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only