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Plasmid#108484Purposeinducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
H9 F42A pAcGP67a
Plasmid#41810DepositorInsertH9 Super-2 (IL2 Human)
UseTags8x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-YA/VD-GFP
Plasmid#60703Purposemammalian expression of rat Cx43 YA/VD mutant fused to GFPDepositorInsertCx43 (Gja1 Rat)
UseTagsEGFPExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS-CD64-2
Plasmid#109191PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFull-length engineered CD64, derived from human CD64
UseTagsTwin-StrepExpressionInsectMutationengineered for efficient expression, see publicat…PromoterPolyhedrinAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK JunD-HA neo
Plasmid#58515PurposeLentiviral expression vector for an inducible HA-tagged mouse JunDDepositorInsertJunD (Jund Mouse)
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-SpyTag-C1C2-mCherry
Plasmid#69833PurposeLentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.DepositorInsertSpyTag-C1C2
UseLentiviralTagsTrafficking signal and mCherryExpressionMammalianMutationInserted SpyTag after the signal peptide of C1C2.PromoterCamKIIaAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 VAMP2 R125TAG
Plasmid#69876Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2-GFPDepositorInsertVAMP2-GFP (Vamp2 Rat)
UseSynthetic BiologyTagsGFPExpressionMammalianMutationAmber stop codon in the linker between VAMP2 and …PromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(721-1176aa)
Plasmid#139690PurposeExpresses N-teminal Flag tagged aa 721-1176 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
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HcRed-pcw107-V5
Plasmid#64647Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-YWAA-pGK-HYG
Plasmid#107377PurposeLentiviral expression of myc-YWAADepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationCRBN-Y383A/W385APromoterUbcPAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV1-B
Plasmid#74974PurposeshETV1 shRNADepositorInsertETV1 (ETV1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-NoTag
Plasmid#105767PurposeControl vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.DepositorTypeEmpty backboneUseFlp-in competentTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mTagBFP-TEV
Plasmid#105772PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsmTagBFP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T12/35A
Plasmid#47938Purposeexpresses Myc tagged human MOB1 containing T12A and T35A mutationsDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT12A and T35APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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LXSN-Cx43-S279/282A
Plasmid#60713Purposeretroviral expression of rat Cx43 S279/282A mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS279/282APromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279/282D
Plasmid#60715Purposeretroviral expression of rat Cx43 S279/282D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS279/282DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNM2_Dynamin-N
Plasmid#110008PurposeProtein expression and purification of DNM2_Dynamin-NDepositorInsertDNM2_Dynamin-N (DNM2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-FLAG-TEV
Plasmid#105768PurposeExpression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.DepositorTypeEmpty backboneUseFlp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AR_Hormone-recep
Plasmid#109847PurposeProtein expression and purification of AR_Hormone-recepDepositorInsertAR_Hormone-recep (AR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-mAPPLE
Plasmid#60696Purposeretroviral expression of rat Cx43 fused to mAPPLEDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsmAPPLEExpressionMammalianMutationPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-YA/VD
Plasmid#60699Purposemammalian expression of rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseTagsExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CLTC_Clathrin
Plasmid#109997PurposeProtein expression and purification of CLTC_ClathrinDepositorInsertCLTC_Clathrin (CLTC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Sos1 (PRM) - Cys (XSB364)
Plasmid#78506PurposeConstruct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labelingDepositorInsertSos1 -Cys (PRM) (SOS1 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-RvCAHS8-GS
Plasmid#192461PurposeExpression of RvCAHS8-GS_linker-AcGFP1 in mammalian cellsDepositorInsertCAHS8
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-YA/VD
Plasmid#60700Purposeretroviral expression of rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279D
Plasmid#60707Purposeretroviral expression of rat Cx43 S279D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS279DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S282A
Plasmid#60709Purposeretroviral expression of rat Cx43 S282A mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS282APromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S282D
Plasmid#60711Purposeretroviral expression of rat Cx43 S282D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS282DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GPR155_DEP
Plasmid#109842PurposeProtein expression and purification of GPR155_DEPDepositorInsertGPR155_DEP (GPR155 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABLIM1_LIM
Plasmid#109880PurposeProtein expression and purification of ABLIM1_LIMDepositorInsertABLIM1_LIM (ABLIM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT3003
Plasmid#122537PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::pisy-1 (pisy-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3014
Plasmid#122540PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::rab-7 (rab-7 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESR1_Hormone-recep
Plasmid#109906PurposeProtein expression and purification of ESR1_Hormone-recepDepositorInsertESR1_Hormone-recep (ESR1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only