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Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF20
Plasmid#52645Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF20
UseTagsExpressionMammalianMutationD362N/D366N mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBL725
Plasmid#140395PurposeExpresses SmtB protein under the control of T7-lacO promoterDepositorInsertT7-lacO-SmtB-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Fast-GCaMP-EF16
Plasmid#52642Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF16
UseTagsExpressionMammalianMutationD431N/D433N/D435N/E442A mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF19
Plasmid#52644Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF19
UseTagsExpressionMammalianMutationT328D/T330D mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBL717
Plasmid#140387PurposeExpresses MobR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MobR-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
UseTagsHRV3C cleavage site and StrepII-tag withExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13, MG-26-82)
Plasmid#110746PurposeBacterial expression of human USP30 (construct 13)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350S, I353E + insertion deletion: 64-178;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETa(+) HpCsd3 (42-403)
Plasmid#71285PurposeExpresses Csd3 from Helicobacter pyloriDepositorInsertHP0506 (HP0506 )
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-GFP-Patronin CC 868–1457
Plasmid#59053Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-C
Plasmid#190966PurposeTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
SENP2M497A
Plasmid#61088Purposecleave the SUMO off when use PETSUMO vector for express fusion proteinDepositorInsertSENP2M497A catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationM497A; Catalytic domain is residues 364-589PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SETDB1
Plasmid#25240PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETDB1, SETDB1_16 (SETDB1 Human)
UseTags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXC13(human)-pET
Plasmid#8679DepositorInsertHOXC13 (HOXC13 Human)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Coomassie blue stainingPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK357
Plasmid#71568PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBL711
Plasmid#140381PurposeExpresses OtrR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-OtrR-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
DNMT3B (3QKJ)
Plasmid#32044PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDNMT3B (DNMT3B Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPWWP domain only (amino acids 293-442)PromoterAvailable sinceFeb. 22, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dom34(full length)
Plasmid#19098DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET:GACG:GFP
Plasmid#60735PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ WT
Plasmid#129647PurposeExpresses the mature metQ gene from Neisseria meningitides, without the signal sequenceDepositorInsertmetQ Gene
UseTags10x Histidine Tag, Enterokinase, and pelB signal …ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
HDGF2
Plasmid#32021PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHDGF2 (HDGFL2 Human)
UseTagsHisExpressionBacterialMutationPWWP domain only (amino acid residues 1-93)PromoterT7Available sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET:GCAT:GFP
Plasmid#60736PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (143 - 575)
Plasmid#110751PurposeBacterial expression of PhPINK1 (crystallography)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only