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pMC0x_gRNA_Pos5b1_Ptet

Plasmid

#179341

Purpose

gRNA Position Vector 5b1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a2_Ptet

Plasmid

#179340

Purpose

gRNA Position Vector 5a2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLCKO_EIF3D_sgRNA_1

Plasmid

#74187

Purpose

lentiviral vector expressing sgRNA targeting EIF3D

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

EIF3D sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLCKO_EIF3D_sgRNA_4

Plasmid

#74188

Purpose

lentiviral vector expressing sgRNA targeting EIF3D

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

EIF3D sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA3_IL1RN

Plasmid

#64151

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos8_AmpR

Plasmid

#119151

Purpose

Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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XX>sgRNA F+E

Plasmid

#87543

Purpose

Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G8_Dual_sgRNA

Plasmid

#178104

Purpose

Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G8 sgRNA + user-specified sgRNA

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No4

Plasmid

#194899

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M7NS_AmpR

Plasmid

#119157

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M2S_AmpR

Plasmid

#119154

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_pos7_AmpR

Plasmid

#119150

Purpose

Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos5_AmpR

Plasmid

#119148

Purpose

Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA2_IL1RN

Plasmid

#64142

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_IL1RN

Plasmid

#64152

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_SA-2x

Plasmid

#79369

Purpose

Assays activity of transcriptional activators fused to SA Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGGC_RT_Sa_sgRNA

Plasmid

#136968

Purpose

Goldengate/Greengate module for genome editing, using the Sa guideRNA.

Depositor

Klaus Theres

Insert

Sa guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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