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pET-UNC60B

Plasmid

#48740

Purpose

Bacterial expression of UNC-60B (a C. elegans ADF/cofilin)

Depositor

Shoichiro Ono

Article

Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Insert

C. elegans UNC-60B cDNA (unc-60 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET-UNC60A

Plasmid

#48739

Purpose

Bacterial expression of UNC-60A (a C. elegans ADF/cofilin)

Depositor

Shoichiro Ono

Article

Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Insert

C. elegans UNC-60A cDNA (unc-60 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Use

Tags

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pPD95.75-cYAP-1

Plasmid

#66857

Purpose

Generate transgenic C.elegans with cYAP-1 fused to GFP

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

C.elegans YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

C. elegans 5'UTR

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
RGS-6xHis-BLMP-1-pcDNA3.1-

Plasmid

#52514

Purpose

expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

blmp-1 (blmp-1 Nematode)

Use

Tags

6xHis

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-LIN-29-pcDNA3.1-

Plasmid

#52515

Purpose

expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Lin-29 b (lin-29 Nematode)

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

wt (changed coding sequence in 5' (5th codon…

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFM111

Plasmid

#40157

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1N:FLAG (par-1 Nematode)

Use

Tags

Flag and GST

Expression

Bacterial

Mutation

Contains aa 1-964

Promoter

tac

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM114

Plasmid

#40160

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N+S251A (par-3 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa 1-364); S251A

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM116

Plasmid

#40162

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C+S950A (par-3 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa840-1268); S950A

Promoter

tac

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pMDJ231 - Phsp Cas9

Plasmid

#191382

Purpose

Heat shock inducible Cas9 expression in C. elegans.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Phsp-16.41 Cas9 gpd-2 tagRFP-t

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Phsp-16.41

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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KG#371

Plasmid

#110882

Purpose

Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

Inserts

unc-129 promoter
CTNS-1a-mCherry cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Use

Tags

intron-mCherry

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFM112

Plasmid

#40158

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1C:FLAG (par-1 Nematode)

Use

Tags

GST and modified Flag

Expression

Bacterial

Mutation

Contains aa 966-1192

Promoter

tac

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only