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pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_MAPT:EGFP

Plasmid

#92196

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-MAPT-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his

Plasmid

#24055

Depositor

Jonathan Backer

Article

Yan et al Biochem J. 2009 Feb 1. 417(3):747-55.

Insert

PIK3C3 and PIK3R4 (PIK3C3, PIK3R4 Human)

Use

Bicistronic

Tags

Myc (hVps34) and V5/his (hVps15)

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pGEMT-Mef2c-tPT2A-GFP

Plasmid

#111771

Purpose

Bicistronic construct: Co-express two genes by 2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Insert

Mef2c-tPT2A-GFP (Mef2c Mouse)

Use

Cloning intermediate

Tags

GFP

Expression

Mutation

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217513

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131003

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217515

Purpose

Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hFTSJ3-FLAG

Plasmid

#165463

Purpose

Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)

Use

Retroviral; Gateway

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/MSV, 5'LTR

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-p18Ink4c-IRES-GFP

Plasmid

#52220

Purpose

retroviral bicistronic expression of p18Ink4c and GFP

Depositor

Martine Roussel

Article

Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

Insert

Ink4c (Cdkn2c Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pTol2-PcyA-IRES-HO1

Plasmid

#131787

Purpose

Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PcyA and HO1

Use

Synthetic Biology; Zebrafish tol2 transposon

Tags

MTS (Mitochondrial Targeting Signal from subunit …

Expression

Mammalian

Mutation

Promoter

hsp70

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

Plasmid

#233628

Purpose

Expression of bicistronic reporter with TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

Plasmid

#233629

Purpose

Expression of bicistronic reporter with mutated TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

AGAAGG in TOP motif

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Plasmid

#217514

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pILGFP4QE2Crimson

Plasmid

#218280

Purpose

Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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