Addgene

We narrowed to 1,712 results for: FAST

Showing: 221 - 240 of 1712 results

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pFastBac His HA GNU WT

Plasmid

#112994

Purpose

Baculovirus expression of His/HA-tagged Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

gnu (gnu Fly)

Use

Tags

His/HA

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS12

Plasmid

#52650

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS12

Use

Tags

Expression

Mammalian

Mutation

deleted R40/R41/K42 from GCaMP3

Promoter

T7

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac-LacI-LAP

Plasmid

#114034

Purpose

Production of baculovirus with LacI-LAP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Mel18 flag

Plasmid

#1969

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Mel18 (Pcgf2 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-EF09

Plasmid

#52640

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF09

Use

Tags

Expression

Mammalian

Mutation

D366N mutation from GCaMP3 sequence

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Use

Tags

His6-tev

Expression

Insect

Mutation

K70A

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001144738)

Plasmid

#77876

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001145485)

Plasmid

#77877

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF15

Plasmid

#52641

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF15

Use

Tags

Expression

Mammalian

Mutation

D431N/D433N/D435N mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-long-CFAST10

Plasmid

#233598

Purpose

Expression of CFAST10 on the OMM membrane after a long linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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ER-long-RspA-NFAST

Plasmid

#233600

Purpose

Expression of RspA-NFAST on the ER membrane after a 55 aminoacid linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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STIM1-RspA-NFAST

Plasmid

#233606

Purpose

Expression of hSTIM1-RspA-NFAST

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

STIM1 (STIM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX R139A

Plasmid

#192058

Purpose

Expression vector for SOX (ORF37) R139A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Use

Tags

Expression

Insect

Mutation

R139A

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Use

Tags

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Use

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

Plasmid

#187723

Purpose

Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 1-418+582-975 (NEDD4L Human)

Use

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 419-581

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_W1-L-W2-HECT

Plasmid

#187724

Purpose

Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 153-418+582-975 (NEDD4L Human)

Use

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 1-152 and 419-581

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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