Addgene

We narrowed to 16,252 results for: Emb;

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PNCA-mCherry

Plasmid

#67790

Purpose

Expression of MMLV proviral DNA-mCherry. Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1

Insert

MMLV-LTR-mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
InterTag-BFP

Plasmid

#186901

Purpose

Cell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-PDGFR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 1

Plasmid

#102709

Purpose

Provides the Middle-to-End linker 1 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 2

Plasmid

#102710

Purpose

Provides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 3

Plasmid

#102711

Purpose

Provides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4A

Plasmid

#102712

Purpose

Provides the End-to-End linker 4A which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Γ

Plasmid

#102714

Purpose

Provides the End-to-End linker 4Γ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Δ

Plasmid

#102715

Purpose

Provides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#102680

Purpose

Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:amilCP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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p1990 SV40-LMP1

Plasmid

#26654

Depositor

Bill Sugden

Article

Kaykas et al EMBO J. 2001 Jun 1. 20(11):2641-54.

Insert

Latent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4B

Plasmid

#102713

Purpose

Provides the End-to-End linker 4B which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Use

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Promoter

Available since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH_blast_MCS_Nard_GFP_D50

Plasmid

#167339

Purpose

Expresses GFP fused to progerin (d50) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Progerin

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCitrine

Plasmid

#155220

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH5

Plasmid

#199771

Purpose

Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH5

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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