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Showing: 221 - 240 of 336 results

  1. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid
    #105801
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: FLAG-3C; ORF2: mClover3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105801-plasmid-map-sequence-id-203395

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  5. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid
    #105803
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-FLAG; ORF2: TEV-mClover3
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105803-plasmid-map-sequence-id-203399

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  7. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Kai Wang
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    215544-plasmid-map-sequence-id-424979

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  15. CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid
    #165435
    Purpose
    Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165435-plasmid-map-sequence-id-325982

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  19. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid
    #165437
    Purpose
    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…
    Promoter
    CMV
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    165437-plasmid-map-sequence-id-325980

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  20. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid
    #165436
    Purpose
    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…
    Promoter
    CMV
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    165436-plasmid-map-sequence-id-325943

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Showing: 221 - 240 of 336 results