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attB-GFP-Tet1d834-1053-Poly(A)

Plasmid

#65561

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1053)

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL8A-no stop

Plasmid

#67333

Purpose

hArl8a without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Plasmid

#200793

Purpose

Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

1-914; Δ505-610

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852R pc3914

Plasmid

#201704

Purpose

Expresses of GFP-tagged mTet1s_K852R in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852R (Tet1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to arginine

Promoter

CAG

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852E pc3915

Plasmid

#201705

Purpose

Expresses of GFP-tagged mTet1s_K852E in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852E (Tet1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to glutamate

Promoter

CAG

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pmch-mTet1-ZF-CD pc3956

Plasmid

#201709

Purpose

Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1-ZF_CD (Tet1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Zinc finger domain of Tet1 fused to its catalytic…

Promoter

CAG

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Use

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147768)

Plasmid

#76194

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147701)

Plasmid

#76191

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001145915)

Plasmid

#76192

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PEX3

Plasmid

#227303

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PEX3 (PEX3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001145643)

Plasmid

#76193

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1-S127A

Plasmid

#213584

Purpose

Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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