Showing: 221 - 240 of 291 results
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Plasmid#76193Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3CBDepositorInsertPIK3CB (PIK3CB Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
PIK3CB gRNA (BRDN0001147768)
Plasmid#76194Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3CBDepositorInsertPIK3CB (PIK3CB Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1-S127A/S397A
Plasmid#213585PurposeDoxycycline inducible expression of YAP1-S127A/S397A cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationS127A, S397APromoterAvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1-S127A/S397A/S94A
Plasmid#213586PurposeDoxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationS127A, S397A, S94APromoterAvailabilityAcademic Institutions and Nonprofits only -
pInducer-TAZ-S89A/S311A/S51A
Plasmid#213590PurposeDoxycycline inducible expression of TAZ-S89A/S311A/S51A cDNA in mammalian cellsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTags2xHAExpressionMammalianMutationS89A, S311A, S51APromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV
Plasmid#102421PurposeInducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vectorDepositorInsertTet1 (Tet1 Mouse)
UseTagsHAExpressionMammalianMutationModified at the Dharmacon SMARTpool siRNA #2 targ…PromoterTetO-CMVAvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF5A*-strep
Plasmid#120163PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidinDepositorInsertkinesin family member 5A (Kif5a Mouse)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF5A: 1-559 aaPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA1
Plasmid#131139PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCI-Puro_mouse_Adar2_Tv1
Plasmid#158111Purposeexpresses mADAR2 shortest transcript, puromycin selectionDepositorInsertadar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A
Plasmid#166954PurposeExpresses FLAG-tagged KIF5A protein in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA
Plasmid#174900Purposeamber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertCRFR1 (Crhr1 Rat)
UseTagsHAExpressionMammalianMutationA95TAGPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro
Plasmid#183409PurposepiggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cellsDepositorInsertdCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro
Plasmid#183410PurposepiggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cellsDepositorInsertKRAB-dCas9-ecDHFR
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-ILF3-ts1
Plasmid#174265PurposeILF3 knockdownDepositorInsertILF3 shRNA (ILF3 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-ILF3-ts2
Plasmid#174266PurposeILF3 knockdownDepositorInsertILF3 shRNA (ILF3 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-ILF3-ts3
Plasmid#174267PurposeILF3 knockdownDepositorInsertILF3 shRNA (ILF3 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CB_WT
Plasmid#82221PurposeGateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CB (PIK3CB Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CB_p.A1048V
Plasmid#81533PurposeGateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CB (PIK3CB Human)
UseGateway entry vectorTagsExpressionMutationA1048VPromoterNoneAvailabilityAcademic Institutions and Nonprofits only
Showing: 221 - 240 of 291 results