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Plasmid#72001PurposeExpresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(S)-AP-His
Plasmid#72018PurposeExpresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(S)-AP-His
Plasmid#72014PurposeExpresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3b (Sema3b Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-JAM-A
Plasmid#112965PurposeTo express feline Jam-A transiently in cell lines that do not naturally express the receptor.DepositorInsertJunctional Adhesion Molecule type A (JAM-A) (F11R Felis catus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc rat S-SCAM alpha
Plasmid#40213DepositorInsertS-SCAM (Magi2 Rat)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 S HexaPro
Plasmid#154754PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variantDepositorInsertSpike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSCD-Casp3-L-tev-S
Plasmid#226230PurposeExpress TEVP-cleavable caspase-3DepositorInsertCaspase-3 (CASP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas ServiceAAV5InserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-YFP
Plasmid#55781PurposeThis G protein alpha-s construct contains internal insertions of YFP and the EE epitope.DepositorInsertalpha-s-EE YFP (Gnas Rat)
UseTagsEYFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationMet was substituted for Gln69 in EYFP (Clontech).…PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PM-Ras-LOCKR-S
Plasmid#216498PurposeFRET-based reporter for Ras activity, plasma membrane localizedDepositorInsertPM-Ras-LOCKR-S
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Golgi-Ras-LOCKR-S
Plasmid#216499PurposeFRET-based reporter for Ras activity, Golgi localizedDepositorInsertGolgi-Ras-LOCKR-S
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 ER-Ras-LOCKR-S
Plasmid#216500PurposeFRET-based reporter for Ras activity, ER localizedDepositorInsertER-Ras-LOCKR-S
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito-Ras-LOCKR-S
Plasmid#216501PurposeFRET-based reporter for Ras activity, mitochondria localizedDepositorInsertMito-Ras-LOCKR-S
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-OMICRON
Plasmid#180593PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertSpike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S
Plasmid#149329PurposeGateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSH1/S-Fvls-p30Casp9-E
Plasmid#15272DepositorInsertFKBP12(V36)-p30Caspase9 (CASP9 Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFKBP12 F36 to VTruncated Caspase9 (135-416; lacki…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.5
Plasmid#213069PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variantDepositorInsertS-GSAS-Omicron.BA5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-S-LexA::p65
Plasmid#133564PurposeSwap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S in pAR9 (rep/cap)
Plasmid#174539Purposeencodes AAV-S capsid for efficient gene delivery to the mammalian cochleaDepositorInsertVP1 of AAV9 containing the AAV-S 7-mer peptide insert
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only