Skip to main content
Addgene
Showing: 551 - 600 of 4441 results

  2. pUC19-Trigger-Bottom

    Plasmid
    #87317
    Purpose
    Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87317-plasmid-map-sequence-id-174671

    Enlarge

  11. pUC19 PKC eta

    Plasmid
    #8431
    Depositor
    Frederic Mushinski
    Insert
    Mouse Protein Kinase C eta (Prkch Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  13. pUC19-P70a-Top-T

    Plasmid
    #87312
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli system "Top" transcript
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    Jan. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    87312-plasmid-map-sequence-id-201363

    Enlarge

  19. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid
    #219989
    Purpose
    betaglobin-5'UTR-NanoLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    betaglobin-5'UTR-NanoLuc (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219989-plasmid-map-sequence-id-436835

    Enlarge

  20. pUC57-T7-PAB1-NLuc

    Plasmid
    #219984
    Purpose
    PAB1-5'UTR-NLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    PAB1-5'UTR-NLuc (PAB1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219984-plasmid-map-sequence-id-436830

    Enlarge

  22. pUC-tracrRNA-PMtU6-26

    Plasmid
    #209191
    Purpose
    Cloning of sgRNAs for expression in plants
    Depositor
    Christian Staehelin
    Insert
    MtU6-26 SnRNA promoter
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209191-plasmid-map-sequence-id-419151

    Enlarge

  23. pUC-tracrRNA-PMtU6-1

    Plasmid
    #209190
    Purpose
    Cloning of sgRNAs for expression in plants
    Depositor
    Christian Staehelin
    Insert
    MtU6-1 SnRNA promoter
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209190-plasmid-map-sequence-id-419127

    Enlarge

  24. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

    Enlarge

  26. pUC19-Ï€-EB1_IRES-mCherry

    Plasmid
    #197427
    Purpose
    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197427-plasmid-map-sequence-id-388174

    Enlarge

  27. pUC19-π-EB1_EF1α-mCherry

    Plasmid
    #197429
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197429-plasmid-map-sequence-id-388078

    Enlarge

  28. pUC19-π-EB1_EF1α-EGFP

    Plasmid
    #197430
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    EGFP, GCN4 leucine zipper, LOV2, and Zdk1
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197430-plasmid-map-sequence-id-388175

    Enlarge

  29. pUC57-mini v2-HF24_F4

    Plasmid
    #195718
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#4 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195718-plasmid-map-sequence-id-385728

    Enlarge

  30. pUC57-mini v2-HF24_F20

    Plasmid
    #195734
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#20 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195734-plasmid-map-sequence-id-387873

    Enlarge

  31. pUC57-mini v2-HF24_F5

    Plasmid
    #195719
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#5 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195719-plasmid-map-sequence-id-387872

    Enlarge

  32. pUC57-mini v2-HF24_F12

    Plasmid
    #195726
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#12 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195726-plasmid-map-sequence-id-387868

    Enlarge

  33. pUC57-mini v2-HF24_F3

    Plasmid
    #195717
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#3 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195717-plasmid-map-sequence-id-387867

    Enlarge

  34. pUC57-mini v2-HF24_F24

    Plasmid
    #195738
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#24 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195738-plasmid-map-sequence-id-387865

    Enlarge

  35. pUC57-mini v2-HF24_F7

    Plasmid
    #195721
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#7 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195721-plasmid-map-sequence-id-387861

    Enlarge

  36. pUC57-mini v2-HF24_F8

    Plasmid
    #195722
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#8 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195722-plasmid-map-sequence-id-387859

    Enlarge

  37. pUC57-mini v2-HF24_F14

    Plasmid
    #195728
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#14 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195728-plasmid-map-sequence-id-387858

    Enlarge

  38. pUC57-mini v2-HF24_F13

    Plasmid
    #195727
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#13 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195727-plasmid-map-sequence-id-385601

    Enlarge

  39. pUC57-mini v2-HF24_F22

    Plasmid
    #195736
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#22 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195736-plasmid-map-sequence-id-385599

    Enlarge

  40. pUC57-mini v2-HF24_F10

    Plasmid
    #195724
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#10 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195724-plasmid-map-sequence-id-385596

    Enlarge

  41. pUC57-mini v2-HF24_F6

    Plasmid
    #195720
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#6 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195720-plasmid-map-sequence-id-385595

    Enlarge

  42. pUC57-mini v2-HF24_F15

    Plasmid
    #195729
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#15 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195729-plasmid-map-sequence-id-385594

    Enlarge

  43. pUC57-mini v2-HF24_F17

    Plasmid
    #195731
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#17 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195731-plasmid-map-sequence-id-385593

    Enlarge

  44. pUC57-mini v2-HF24_F19

    Plasmid
    #195733
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#19 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195733-plasmid-map-sequence-id-385592

    Enlarge

  45. pUC57-mini v2-HF24_F2

    Plasmid
    #195716
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#2 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195716-plasmid-map-sequence-id-385591

    Enlarge

  46. pUC57-mini v2-HF24_F11

    Plasmid
    #195725
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#11 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195725-plasmid-map-sequence-id-385589

    Enlarge

  47. pUC57-mini v2-HF24_F16

    Plasmid
    #195730
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#16 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195730-plasmid-map-sequence-id-385583

    Enlarge

  48. pUC57-mini v2-HF24_F18

    Plasmid
    #195732
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#18 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195732-plasmid-map-sequence-id-385582

    Enlarge

  49. pUC57-mini v2-HF24_F1

    Plasmid
    #195715
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#1 of a 24 fragment split in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195715-plasmid-map-sequence-id-385580

    Enlarge

  50. pUC57-mini v2-HF24_F9

    Plasmid
    #195723
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#9 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195723-plasmid-map-sequence-id-385578

    Enlarge

Showing: 551 - 600 of 4441 results