Addgene

We narrowed to 723 results for: ucoe

Showing: 331 - 360 of 723 results

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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LV-sgRNA-mCherry-Puro sgPIDD

Plasmid

#211528

Purpose

Deletes PIDD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgRPS3

Plasmid

#211526

Purpose

Facilitate knock-in of RPS3-Keima fusion protein

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgRPS3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 hygro sgUCK1

Plasmid

#211524

Purpose

Deletes UCK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pL0-MjcDOPA5GT

Plasmid

#162530

Purpose

Mirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

cDOPA5GT

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Sln

Plasmid

#105216

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Sln (Sln Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR SS287AA

Plasmid

#72703

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA

Promoter

ubiquitin

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
HA-Foxo1 (pCMV5)

Plasmid

#12142

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 (Foxo1 Mouse)

Use

Tags

HA and Myc

Expression

Mammalian

Mutation

Wild-type

Promoter

CMV

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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ISceI-GR-RFP

Plasmid

#17654

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)

Use

Tags

mRFP

Expression

Mammalian

Mutation

C655G mutation in the glucocorticoid receptor (se…

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-cIAP2/pRK5

Plasmid

#27973

Depositor

Xiaolu Yang

Article

Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

Insert

CIAP2 (BIRC3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-GFP

Plasmid

#200093

Purpose

WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-FLAG

Plasmid

#200094

Purpose

WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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plenti6-SLC7A11/xCT-V5

Plasmid

#170427

Purpose

Eepresses SLC7A11 (also named xCT) in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Foxo1

Plasmid

#17551

Depositor

Domenico Accili

Article

Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

Insert

pEGFP-N1 Foxo1 (Foxo1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only

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FLHKI-pGFPN3

Plasmid

#21917

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKI in pGFP-N3 (HK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

This is full length human HKI in pGFP-N3

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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hSirt3-RFP

Plasmid

#216806

Purpose

Human Sirt3-RFP driven by the CaMKII promoter

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (SIRT3 Human)

Use

Lentiviral

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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FLHKII-pGFPN3

Plasmid

#21920

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-TetO-PM

Plasmid

#197387

Purpose

To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral; Teto

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPAP_BGL1

Plasmid

#183735

Purpose

Expresses Aspergillus aculeatus β-glucosidase (BGL1)

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

β-glucosidase (AaBGL1)

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1 promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast coUGP2

Plasmid

#211531

Purpose

Expresses codon optimized UGP2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

UGP2 (UGP2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-p53/pRK5

Plasmid

#39237

Depositor

Xiaolu Yang

Article

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

p53 (TP53 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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SEH1L in pRK5

Plasmid

#154216

Purpose

Untagged SEH1L cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

SEH1L (SEH1L Human)

Use

Tags

NONE

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBS MCK promoter

Plasmid

#12528

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Insert

MCK promoter (Ckm Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pISH-Emx1-SR

Plasmid

#105214

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Emx1 (Emx1 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE repeats (dox off)

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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GFP HDAC5 WT

Plasmid

#32211

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
FLAG HDAC5 WT

Plasmid

#32213

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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