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pET28a-DONSON-WM

Plasmid

#208798

Purpose

Xenopus laevis His6-Myc-DONSON (W234L, M463T)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

W234L; M463T

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-3A

Plasmid

#208800

Purpose

Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

V473A; R476A; Y481A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-DONSON-S437A

Plasmid

#208803

Purpose

Xenopus laevis His6-Myc-DONSON (S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-DONSON-ΔD1

Plasmid

#208804

Purpose

Xenopus laevis His6-DONSON (1-155aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His

Expression

Bacterial

Mutation

ΔD1, deletion of 1-155aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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XlMT Xenopus laevis Metallothionein

Plasmid

#200293

Purpose

Bacterial expression plasmids for production of recombinant Xenopus laevis Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_NBT:Cas9green; erbb2_gRNA171

Plasmid

#199337

Purpose

pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

NBT
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCav1 in pT3TS-Dest

Plasmid

#194293

Purpose

In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin (cav1.S Frog)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa_R551Q:polyA

Plasmid

#193013

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa R551Q (NRG1 Human)

Use

Transposon transgenesis

Tags

Expression

Mammalian

Mutation

ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1-myc

Plasmid

#185511

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

deletion of IDILGE in acidic blob

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1

Plasmid

#185510

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

deletion of IDILGE in acidic blob

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-EnR

Plasmid

#185497

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Yan et al Dev Biol. 2009 May 1;329(1):80-95. doi:

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

fusion of winged helix domain with EnR domain

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB4

Plasmid

#185513

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

IDILGE replaced with AAAAAA

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-AB2

Plasmid

#185512

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

IDIL replaced with AAAAAA

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-deltaAB-myc

Plasmid

#185503

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

acidic blob (aa 21-33) deleted

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaAB

Plasmid

#185502

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

acidic blob (aa 21-33) deleted

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-foxd4l1.1

Plasmid

#185493

Purpose

ISH probe for X laevis foxd4

Depositor

Sally Moody

Article

Sullivan et al Dev Biol. 2001 Apr 15. 232(2):439-57.

Insert

foxd4l1.1 S homeolog

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-QR-myc

Plasmid

#185521

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

replaces Q>R upstream of GARQYLNI

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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