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pZ_P-ICL1-eGFP

Plasmid

#126716

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

ICL1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Phhf2-PYC-Tadh1

Plasmid

#127737

Purpose

Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Phhf2

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-ACS1-eGFP

Plasmid

#126715

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

Expression

Yeast

Mutation

Promoter

ACS1

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-ALD-eGFP

Plasmid

#126714

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

Expression

Yeast

Mutation

Promoter

ALD

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-HXT1-eGFP

Plasmid

#126719

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

Expression

Yeast

Mutation

Promoter

HXT1

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-ADH3-eGFP

Plasmid

#126713

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH3

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ALD1

Plasmid

#126711

Purpose

yeast plasmid complementing ALD1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

ald1

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ADH3

Plasmid

#126710

Purpose

yeast plasmid complementing ADH3 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh3

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pK_lacO-cP-AOX1-sAR

Plasmid

#126743

Purpose

yeast plasmid overexpressing CPR and sLovA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA,cpr

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Use

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C

Plasmid

#60398

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptide

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

Plasmid

#60405

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pDSM-bc-Prps3-CAD-Tcyc1

Plasmid

#127728

Purpose

Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Prps3

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Peft2-CAD-Tklura3

Plasmid

#127729

Purpose

Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Peft2

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSM-bc-Psmtef1-CAD-Tyhi9

Plasmid

#127730

Purpose

Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Psmtef1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Psptdh3-ACO-Tagtef1

Plasmid

#127734

Purpose

Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Psptdh3

Available since

Availability

Academic Institutions and Nonprofits only

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