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Plasmid#195619PurposeExpresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutationDepositorInsertI-WcaI
UseTagsExpressionBacterialMutationA2030T, A2031TPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(ETNPPL)
Plasmid#192264PurposeCoding sequence of mouse ETNPPL is inserted in pET-15b vectorDepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His
Plasmid#195610PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tagDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT825C, C900G, A901C, A903C, T1131G, T1137C, T1227…PromoterT7Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC
Plasmid#195607PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoterDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationC552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…PromoterT7Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmNot1_1147-1386_P
Plasmid#147175PurposeBacterial Expression of DmNot1_1147-1386DepositorInsertDmNot1_1147-1386 (Not1 Fly)
UseTagsExpressionBacterialMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmNot1_1147-1717_P
Plasmid#147176PurposeBacterial Expression of DmNot1_1147-1717DepositorInsertDmNot1_1147-1717 (Not1 Fly)
UseTagsExpressionBacterialMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmNot3_677-844_P
Plasmid#147177PurposeBacterial Expression of DmNot3_677-844DepositorInsertDmNot3_677-844 (Not3 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsNot1iso1_1085-2376_O
Plasmid#147081PurposeBacterial Expression of HsNot1iso1_1085-2376DepositorInsertHsNot1iso1_1085-2376 (CNOT1 Human)
UseTagsExpressionBacterialMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsNot1iso1_1597-2376_O
Plasmid#147082PurposeBacterial Expression of HsNot1iso1_1597-2376DepositorInsertHsNot1iso1_1597-2376 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsNot1iso1_1085-1605_N
Plasmid#147009PurposeBacterial Expression of HsNot1iso1_1085-1605DepositorInsertHsNot1iso1_1085-1605 (CNOT1 Human)
UseTagsExpressionBacterialMutation1 silent mutations compared to the sequence of is…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsPAN3_291-687_N
Plasmid#147013PurposeBacterial Expression of HsPAN3_291-687DepositorInsertHsPAN3_291-687 (PAN3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmPABPC1_J
Plasmid#146640PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-DmPABPC1_501-634_J
Plasmid#146641PurposeBacterial Expression of HsPABPC1_501-634DepositorInsertHsPABPC1_501-634
UseTagsExpressionBacterialMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsPABPC1_271-542_J
Plasmid#146645PurposeBacterial Expression of HsPABPC1_271-542DepositorInsertHsPABPC1_271-542 (PABPC1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-PfAgo_F
Plasmid#146270PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
UseTagsExpressionBacterialMutationone non silent mutation A907G (I303V) and one sil…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 em-blue-shift.2
Plasmid#191901PurposeEncodes a GFP design, em-blue-shift.2DepositorInsertem-blue-shift.2
UseTagsHis TagExpressionBacterialMutationT65S S72A T108I Y145F V150IPromoterT7Available sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN-MutTER
Plasmid#178033PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationChanged T(108)A, E(109)A, R(110)APromoterT7LacIAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP7_rpoA-U
Plasmid#190974PurposeTest the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP7
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP1_rpoA-U
Plasmid#190955PurposeTest the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only