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Plasmid#159901PurposeMutagenesis of ThDepositorInsertTh (Th Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-oG-WPRE-SV40pA
Plasmid#74292PurposepAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVTagsExpressionMammalianMutationchimeric glycoproteinPromoterCAGAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff
Plasmid#107700Purposein vivo voltage imagingDepositorInsertQuasAr3-P2A-CheRiff
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterpCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GAL4-iLight-VP16
Plasmid#170274PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16
UseAAVTagsExpressionMutationSee CommentsPromoterCaMKIIAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLF045: AAV-hSyn-eTsChR-GFP
Plasmid#112280PurposeAAV based broad expression of eTsChR in neuronsDepositorInserteTsChR
UseAAVTagsGFPExpressionMammalianMutationPromoterhSynAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Flpo
Plasmid#55634PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV1InsertsmCherry
Flpo
UseAAVTagsExpressionMammalianMutationPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGfap-R-CaMP1.07-WPRE-SV40
Plasmid#164140PurposeAAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.DepositorInsertR-CaMP1.07
UseAAVTagsExpressionMutationPromoterhGFAPAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas ServiceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV9:cTNT::3Flag-hYAP S127A
Plasmid#86558PurposeTo produce cardiac specific adeno-associated virus expressing activated human YAP.DepositorInsertYAP (YAP1 Human)
UseAAVTags3FlagExpressionMutationSerine 127 was mutated into Alanine, to activate …PromotercTNTAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_U6-ωRNA*_ISDra2-TnpB-mNG
Plasmid#212968PurposeAAV vector for encoding an ISDra2-TnpB driven by EFs promoterDepositorInsertsISDra2-TnpB-T2A-mNG,
ωRNA*
UseAAV and CRISPRTagsNLS and NLS-FLAGExpressionMammalianMutationPromoterEF1a, T7 and U6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-ERT2-iCre-ERT2
Plasmid#178060PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.DepositorInsertERT2-iCre-ERT2
UseAAVTagsExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-WPRE
Plasmid#179460PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-PALMDgRNA1+2-cTnT-Cre -mi122TS
Plasmid#216830Purposecardiomyocytes-specific plasmid, expressing gRNAs targeting Palmd exon7, containing a mi122TS sequence to decrease off-target effects in the liver.DepositorInsertPALMDgRNA
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-mCherry
Plasmid#137132PurposeIntersectional viral expression of mCherry in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-MCS--4x6T
Plasmid#196420PurposeEmpty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV and Cre/Lox; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseCRISPRTagsExpressionMutationPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eNpHR 3.0-EYFP
Plasmid#26971PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibitionDepositorHas ServiceAAV1 and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a)
Plasmid#79672PurposeCre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP (Scn9a jellyfish, Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa-VPR mini.
Plasmid#99685PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoterDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only