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p-Tet-O-Foxp1A-Puro

Plasmid

#221665

Purpose

Expresses Foxp1A in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

Foxp1A (Foxp1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

Plasmid

#206093

Purpose

Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

E314A, E664A, E1370A, E1658A

Promoter

CMV

Available since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

Plasmid

#206098

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

E314A, E664A, E1370A, E1658A

Promoter

CMV/B-actin

Available since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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SITS-Cavin4b-EGFP

Plasmid

#176019

Purpose

Leishmania cell free expression of zebrafish Cavin4b-EGFP. Parton lab clone GRJ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Leishmania cell free expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-Cavin4b-mCherry

Plasmid

#176023

Purpose

Leishmania cell free expression of zebrafish Cavin4b-mCherry. Parton lab clone GWR

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4b-mCherry

Plasmid

#176008

Purpose

Mammalian expression of zebrafish Cavin4b-mCherry. Parton lab clone HYO

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4b(PRDmt)-mCherry

Plasmid

#176009

Purpose

Mammalian expression of zebrafish Cavin4b(PRDmt)-mCherry. Parton lab clone JMD

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4b-EGFP

Plasmid

#176011

Purpose

Mammalian expression of zebrafish Cavin4b-EGFP. Parton lab clone HXM

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Cavin4b_PRDmt

Plasmid

#176017

Purpose

Multisite gateway middle entry clone containing zebrafish Cavin4b_PRDmt with no stop codon. Parton lab clone JLY.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (PRDmt) (cavin4b Zebrafish)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4b in pBluescript(SK+)

Plasmid

#126566

Purpose

For generating RNA probes for in situ hybridisation. Parton lab clone IRZ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-BIN1_tv8

Plasmid

#126574

Purpose

Multisite gateway middle entry vector containing human BIN1 transcriptional variant 8 (muscle specific) with no ATG. Parton lab clone DVA.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

BIN1 (BIN1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-puro

Plasmid

#156169

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-puro

Plasmid

#156170

Purpose

Lentiviral vector for expression of RAP2A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-puro

Plasmid

#156171

Purpose

Lentiviral vector for expression of RAP2A-V12, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-N17-puro

Plasmid

#156172

Purpose

Lentiviral vector for expression of RAP2A-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

Plasmid

#156403

Purpose

Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Gateway cloning

Tags

EGFP

Expression

Mutation

mutant KRAS4B-G12V

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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P3-Lenti-dCas9-Tet1-GFP

Plasmid

#190729

Purpose

Lentiviral construct to express spdCas9-Tet1-GFP.

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

SV40 NLS and T2A-EGFP

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

UbC

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

Plasmid

#176850

Purpose

lentiviral vector for doxycycline-inducible YAP1 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

full length YAP1

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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