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p40 R58Q/E259A
Plasmid#19020DepositorInsertp40phox (NCF4 Human)
UseTagsHisExpressionBacterialMutationR58Q/E259APromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
p40 D293K/E259A
Plasmid#19021DepositorInsertp40phox (NCF4 Human)
UseTagsHisExpressionBacterialMutationD293K/E259APromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
p40 W207R/E259A
Plasmid#19022DepositorInsertp40phox (NCF4 Human)
UseTagsHisExpressionBacterialMutationW207R/E259APromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pCpSRb-4
Plasmid#13726DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHisExpressionBacterialMutationCircularly permuted SRβD210K183 with Precission p…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-DR RecR(K23A/R27A)
Plasmid#12619DepositorInsertDeinococcus radiodurans RecR (recR Deinococcus radiodurans)
UseTagsHisExpressionBacterialMutationchanged Lysine 23 to Alanine, and Arginine 27 to …PromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ecoli PancACe
Plasmid#215712PurposeExpresses acetyl-CoA biosensor in EcoliDepositorInsertPancACe
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBECAb-apr
Plasmid#122001PurposeA cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(E69A)
Plasmid#132392PurposeMutant version of OlyA that senses sphingomyelin without or with cholesterolDepositorInsertOstreolysin A point mutant (E69A)
UseTagsHis6 tagExpressionBacterialMutationMutations: C62S, E69A, C94S, S151CPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(-30)GFP
Plasmid#62936PurposeExpression of 6xHis-(-30)GFP in bacterial cellsDepositorInsert(-30)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CTB
Plasmid#141166PurposeExpresses recombinant Cholera Toxin B SubunitDepositorInsertCTB
UseTagsExpressionBacterialMutationNo secretion signalPromoterT7Available sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
UseTagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET-6xHis-(pos36)GFP
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
UseTags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144B (27-236)
Plasmid#110753PurposeBacterial Expression of RNF144B RBR domainDepositorInsertRNF144B (RNF144B Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
UseTagsSpyCatcher and SpyTagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJBL727
Plasmid#140397PurposeExpresses CsoR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-CsoR-T
UseTags6XHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
ITCH
Plasmid#36197PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH (ITCH Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains HECT domainPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorInsertPRKN (PRKN Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCC25
Plasmid#177319PurposeExpression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coliDepositorInsertTtCutA 240-603
UseTags6xHis-SUMOTagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
tUI
Plasmid#122661PurposeExpress tUI protein in E. coliDepositorUseTagsHis-tagExpressionMutationPromoterT7 promoterAvailable sinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEL1203
Plasmid#196024PurposeExpression/purification of 6xHis-TEV-MlaCDepositorInsertMlaC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
