Skip to main content
Addgene

pET-21a(+)-DR RecR(K23A/R27A)
(Plasmid #12619)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12619 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Deinococcus radiodurans RecR
  • Alt name
    DR RecR
  • Species
    Deinococcus radiodurans
  • Insert Size (bp)
    660
  • Mutation
    changed Lysine 23 to Alanine, and Arginine 27 to Alanine.
  • GenBank ID
    AE000513
  • Entrez Gene
    recR (a.k.a. DR_0198)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a(+)-DR RecR(K23A/R27A) was a gift from SeWon Suh (Addgene plasmid # 12619 ; http://n2t.net/addgene:12619 ; RRID:Addgene_12619)
  • For your References section:

    Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair. Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW. EMBO J. 2004 May 19. 23(10):2029-38. 10.1038/sj.emboj.7600222 PubMed 15116069