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Plasmid#78128Purposeluciferase reporter for miRNA activityDepositorInsertSMAD4 (SMAD4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorInsertZNF598 (ZNF598 Human)
UseTagsTEV-3xFLAGExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas9-Act3.0
Plasmid#178954PurposeIt consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertzCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIL-1L
Plasmid#50016PurposeMammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertRanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-BACE1-mBFP
Plasmid#196697PurposeExpresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertBeta-secretase 1 (BACE1 Human)
UseTagsHA and mBFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-luc2
Plasmid#24343DepositorInsertLuciferase
UseTags5XGAL4ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP
Plasmid#138334PurposeExpresses APOB-Linker-eGFPDepositorInsert5'UTR-ApoB48-Linker-eGFP (APOB Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only