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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Article

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Use

Tags

GFP derived from pMDC107

Expression

Plant

Mutation

Promoter

35s core promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PRPF8

Plasmid

#155853

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF8 (PRPF8 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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VPS4B, S2C

Plasmid

#184806

Purpose

Bacterial expression of VPS4B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS4B (VPS4B Human)

Use

Tags

His6

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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Integrin β8-GFP

Plasmid

#205093

Purpose

Express GFP-tagged human integrin β8 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 8 (ITGB8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Siela Maximova, Mark Guiltinan

Article

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Tags

Expression

Plant

Mutation

Promoter

E12-omega

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn_CapChR2_mScarlet

Plasmid

#188039

Purpose

Expresses CapChR2-mScarlet in mammalian neurons

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Use

AAV

Tags

mScarlet

Expression

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

hSyn

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM3-Flag

Plasmid

#180408

Purpose

lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM3 (TGM3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-LynG

Plasmid

#206867

Purpose

Expression of LynG probe - LynAnchor-eGFP

Depositor

Dirk-Peter Herten

Article

Tashev et al Nat Methods (2024)

Insert

LynG

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Mutation

Contains Lyn amino acids 1-16

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Use

Tags

Expression

Yeast

Mutation

Promoter

10B2 (P1 specific promoter)

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

Plasmid

#66789

Purpose

Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D842V (constitutively active)

Promoter

EF1a

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only
pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101* and PBAD

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SpyCas9

Plasmid

#113034

Purpose

AAV vector for expression of SpyCas9 in mammalian cells

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-EGFP

Plasmid

#14121

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
THOC1

Plasmid

#156081

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC1 (THOC1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.U6.shRNA-Sdc1-1376

Plasmid

#195574

Purpose

Expresses shRNA to knockdown mouse Syndecan-1

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

shRNA, eGFP (Sdc1 Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSF3-microID

Plasmid

#172880

Purpose

expresses microID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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SYMPK

Plasmid

#156071

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SYMPK (SYMPK Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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