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pMOD_A0103

Plasmid

#91000

Purpose

Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUltra-Hot-PC

Plasmid

#184466

Purpose

Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-gfpmut3

Plasmid

#65037

Purpose

ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

10XUAS

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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IKK2-EGFP

Plasmid

#111195

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

IKKbeta (IKBKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ151 - ttTi5605_MCS

Plasmid

#19330

Depositor

Erik Jorgensen

Article

Christian et al

Insert

ttTi5605 targeting region

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-CFP

Plasmid

#90103

Purpose

CFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

CFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SlugABE-NNG

Plasmid

#214356

Purpose

Expresses SlugABE-NNG

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG

Use

AAV

Tags

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFSF13-Fc(DAPA)-AviTag-6xHis

Plasmid

#156611

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFSF13 (TNFSF13 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Fusion of Zika NS2B-NS3 proteins

Plasmid

#204815

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

Frank von Delft, ASAP Discovery

Insert

Zika NS2B-NS3 fusion (orf1ab Zika)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFlc245

Plasmid

#101021

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Use

Tags

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pPSU20

Plasmid

#173829

Purpose

Expresses 20 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb713-HO

Plasmid

#141199

Purpose

Expresses mRhubarb713 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb713
heme oxygenase

Use

Tags

6x-His

Expression

Bacterial

Mutation

R134H, W309R, Q310A compared to iRFP

Promoter

T7

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-LIG3

Plasmid

#215143

Purpose

Expresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

LIG3 (LIG3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSicoR p53

Plasmid

#12090

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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