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Showing: 2321 - 2340 of 2751 results

  3. pRD400

    Plasmid
    #163107
    Purpose
    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_eYFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163107-plasmid-map-sequence-id-321848

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  5. pRD442

    Plasmid
    #168468
    Purpose
    For the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-eYFP-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168468-plasmid-map-sequence-id-331919

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  6. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #119186
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119186-plasmid-map-sequence-id-230533

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  7. Desmoplakin I no force control (F40-based)

    Plasmid
    #119187
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    truncation after aa1945
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119187-plasmid-map-sequence-id-230534

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  8. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119189-plasmid-map-sequence-id-230531

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  9. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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  10. H3S10ph biosensor

    Plasmid
    #120809
    Purpose
    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Threonine 3, Threonine 6, and Threonine 11 are mu…
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120809-plasmid-map-sequence-id-235281

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  11. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al BioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  13. pDZ113

    Plasmid
    #159355
    Purpose
    Expresses sfYFP tagged with ssrA from the Prhl promoter
    Depositor
    Matthew Bennett
    Insert
    sfYFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Prhl
    Available since
    Oct. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159355-plasmid-map-sequence-id-315068

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  14. pDZ114

    Plasmid
    #159357
    Purpose
    Expresses sfYFP tagged with ssrA from the Pcin promoter
    Depositor
    Matthew Bennett
    Insert
    sfYFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Pcin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    159357-plasmid-map-sequence-id-315076

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  15. pDZ115

    Plasmid
    #159356
    Purpose
    Expresses sfYFP tagged with ssrA from the PlLacO1 promoter
    Depositor
    Matthew Bennett
    Insert
    sfYFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PlLacO
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    159356-plasmid-map-sequence-id-315069

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  16. pIC215

    Plasmid
    #69736
    Purpose
    Mammalian Expression of PELP1
    Depositor
    Iain Cheeseman
    Insert
    PELP1 (PELP1 Human)
    Use
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    E927G compared to GenBank reference sequence NP_0…
    Promoter
    Available since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pIC214

    Plasmid
    #69735
    Purpose
    Mammalian Expression of Tousled-like kinase 1 (TLK1)
    Depositor
    Iain Cheeseman
    Insert
    Tousled-like kinase 1 (TLK1) (TLK1 Human)
    Use
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pIC81

    Plasmid
    #63065
    Purpose
    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    PMFI, IMAGE clone 6148250 (PMF1 Human)
    Use
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63065-plasmid-map-sequence-id-104380

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  19. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Promoter
    Available since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  20. pIC79

    Plasmid
    #63063
    Purpose
    Mammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    hNSL1, IMAGE clone 3997280 (NSL1 Human)
    Use
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63063-plasmid-map-sequence-id-104378

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Showing: 2321 - 2340 of 2751 results