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Plasmid#116877PurposeLentiviral transfer vector containing a multible cloning siteDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only
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pSIN4-EF1a-TAL1-IRES-Puro
Plasmid#61065Purposelentiviral vector for TAL1DepositorInsertTAL1 (TAL1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterEF1alphaAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-MIB1-IRES-Puro
Plasmid#140240PurposeLentiviral vector expressing flag-tagged MIB1DepositorInsertFlag-MIB1 (MIB1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)_v2
Plasmid#74408PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GalT-IRES-Puromycin
Plasmid#134862PurposeExpresses EGFP-tagged Golgi markerDepositorInsertbeta 1,4-galactosyltransferase (B4GALT1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 83-398PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-GATA2-IRES-Puro
Plasmid#61063Purposelentiviral vector for GATA2DepositorInsertGATA2 (GATA2 Human)
UseLentiviralTagsHAExpressionMutationPromoterEF1alphaAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-HA-UBE3B(C1036A)-IRES-Puro
Plasmid#107348PurposeExpresses HA tagged UBE3B mutant C1036A in mammalian cells.DepositorInsertUBE3B(C1036A) (UBE3B Human)
UseLentiviralTagsHAExpressionMammalianMutationchanged Cysteine 1036 to AlaninePromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Plasmid#68345PurposeA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expressionDepositorInsertspuromycin
hCas9
UseCRISPR; Flp/frtTagslinked to red flouresent protein gene mKateII and…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro Scr shRNA
Plasmid#30520DepositorInsertScr shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-Flag-IDH1-R132H
Plasmid#62924PurposeRetrovirus expression plasmidDepositorInsertIDH1 (IDH1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationmutation Arg132HisPromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-SNAP-FLAG-CD59
Plasmid#50374PurposeExpress SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cellsDepositorInsertSNAP
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B-copGFP-Puro
Plasmid#107342PurposeExpresses UBE3B fused to copGFP in mammalian cells.DepositorInsertUbiquitin-protein ligase E3B (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti CMV/TO eGFP Puro (w159-1)
Plasmid#17481Purpose3rd gen lentiviral eGFP expression vector, CMV/TO promoter, PuroDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Puro
Plasmid#135683PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-GATA1-IRES-Puro
Plasmid#61062Purposelentiviral vector for GATA1DepositorInsertGATA1 (GATA1 Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1alphaAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinD-IRES-Puro
Plasmid#213114PurposeExpression of untagged MinD in mammalian cellsDepositorInsertMinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
E-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#185987PurposeExpresses SARS-CoV-2 Envelope protein fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 Envelope protein (E SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3RA-PGK-Puro
Plasmid#110846PurposeLentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only